Effect of exogenous insulin on endoplasmic reticulum stress in myocardial tissues during insulin resistance in rabbits undergoing cardiopulmonary bypass
10.3760/cma.j.issn.0254-1416.2019.08.028
- VernacularTitle: 外源性胰岛素对体外循环兔胰岛素抵抗时心肌组织内质网应激的影响
- Author:
Ying ZHANG
1
;
Anqiong MAO
;
Xinzhu QI
;
Yuexin LIU
;
Jiang BIAN
;
Qing LIU
Author Information
1. Department of Anesthesiology, Southwest Medical University Affiliated Traditional Chinese Medical Hospital, Luzhou 646000, China
- Publication Type:Journal Article
- Keywords:
Insulin;
Extracorporeal circulation;
Myocardium;
Insulin resistance;
Endoplasmic recticulum stress
- From:
Chinese Journal of Anesthesiology
2019;39(8):1009-1013
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To evaluate the effect of exogenous insulin on endoplasmic reticulum stress in myocardial tissues during insulin resistance in the rabbits undergoing cardiopulmonary bypass (CPB).
Methods:Forty healthy adult New Zealand white rabbits of both sexes, weighing 2.5-3.0 kg, were divided into 4 groups (n=10 each) using a random number table method: control group (group C), CPB group, CPB plus insulin group (group I) and CPB plus normal saline group (group NS). Group C received no treatment.An insulin resistance model was established in group CPB.Insulin was continuously infused (the infusion rate was adjusted according to the blood glucose) starting from establishing CPB to 1 day after operation in group I. The equal volume of normal saline was given starting from establishing CPB to 1 day after operation in group NS.Blood samples were collected from the left femoral artery, and myocardial tissues obtained before CPB (T1) and at 15, 30 and 60 min after aortic opening (T2-4). The level of blood glucose was determined using oxidase method, the level of glucagon was detected by the radioimmunoassay method, and the insulin resistance index was calculated.The expression of inositol-requiring protein-1α(IRE1α), XBP1 and caspase-12 was measured by Western blot.The expression of IRE1α, XBP1 and caspase-12 mRNA was measured by fluorescent quantitative real-time polymerase chain reaction.Cell apoptosis was detected by TUNEL.
Results:Compared with group C, blood glucose and glucagon levels at T2-4 and insulin resistance index at T4 were significantly increased, and the expression of inositol-requiring protein-1α(IRE1α), XBP1 and caspase-12 protein and mRNA was up-regulated at T4 in CPB, I and NS groups (P<0.05). Compared with group CPB, blood glucose and glucagon levels at T2-4 and insulin resistance index at T4 were significantly decreased, and the expression of IRE1α, XBP1 and caspase-12 protein and mRNA was down-regulated at T4 in group I (P<0.05).
Conclusion:Exogenous insulin significantly improves insulin resistance in myocardial tissues, and the mechanism is related to inhibiting endoplasmic reticulum stress and reducing cell apoptosis in the rabbits undergoing CPB.
