Role of heme oxygenase-1 on lipopolysaccharide-induced activation of NLRP3 inflammasomes in alveolar macrophages of rats
10.3760/cma.j.issn.0254-1416.2019.08.023
- VernacularTitle: HO-1在脂多糖诱导大鼠肺泡巨噬细胞NLRP3炎性小体活化中的作用
- Author:
Lili WU
1
;
Lirong GONG
;
Dinghuan ZHAO
;
Xiangyun LI
;
Shihan DU
;
Jianbo YU
Author Information
1. Department of Anesthesiology, Tianjin Nankai Hospital Nankai Clinical College of Tianjin Medical University Tianjin 300100, China
- Publication Type:Journal Article
- Keywords:
Heme oxygenase-1;
Lipopolysaccharides;
NLR family, pyrin domain-containing 3 protein
- From:
Chinese Journal of Anesthesiology
2019;39(8):989-992
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To evaluate the role of heme oxygenase-1 (HO-1) on lipopolysaccharide (LPS)-induced activation of NOD-like receptor pyrin domain-containing 3 (NLRP3) inflammasomes in alveolar macrophages of rats.
Methods:NR8383 cells of rat alveolar macrophages cultured in vitro were seeded in 96-well plates at a density of 4×104 cells/ml and divided into 4 groups (n=48 each) using a random number table method: control group (group C), group LPS (group L), LPS+ HO-1 siRNA group (group L+ HO-1 siRNA), and LPS+ Con siRNA group (group L+ Con siRNA). The cells were cultured in normal culture atmosphere in group C. NR8383 cells were stimulated with 10 μg/ml LPS in L, L+ HO-1 siRNA and L+ Con siRNA groups.Cells were transfected with HO-1 siRNA at 48 h before stimulation with LPS in group L+ HO-1 siRNA and with Con siRNA at 48 h before stimulation with LPS in group L+ Con siRNA.The cells were collected and incubated for 24 h after stimulation with LPS for measurement of the cell viability, expression of HO-1 and NLRP3 mRNA (by real-time polymerase chain reaction), expression of HO-1, NLRP3, apoptosis-associated speck-like protein containing a caspase recruitment domain (ASC) and caspase-1 (by Western blot), and concentrations of interleukin-1beta (IL-1β) and interleukin-18 (IL-18) in the supernatant (by enzyme-linked immunosorbent assay).
Results:Compared with group C, the cell viability was significantly decreased, the expression of HO-1 and NLRP3 protein and mRNA, ASC and caspase-1 was up-regulated, and the IL-1β and IL-18 concentrations were increased in L, L+ HO-1 siRNA and L+ Con siRNA groups (P<0.05). Compared with group L, the cell viability was significantly decreased, the expression of HO-1 protein and mRNA was down-regulated, and the expression of NLRP3 protein and mRNA, ASC and caspase-1 was up-regulated, and the IL-1β and IL-18 concentrations were increased in group L+ HO-1 siRNA (P<0.05), and no significant change was found in the parameters mentioned above in group L+ Con siRNA (P>0.05).
Conclusion:HO-1 is involved in LPS-induced activation of NLRP3 inflammasomes in alveolar macrophages of rats.
