Effect of Topically Applied Human Epidermal Growth Factor on Stromal Keratocyte Activity in Experimental Corneal Alkali Wounds.
- Author:
Moon Jung KIM
1
;
Roo Min JUN
;
Jang Hyun CHUNG
Author Information
1. Department of Ophthalmology, College of Medicine, Ewha Medical Research Center, Ewha Womans University, Seoul, Korea.
- Publication Type:Original Article
- Keywords:
Corneal alkali wounds;
hEGF;
Keratocyte
- MeSH:
Alkalies*;
Basement Membrane;
Cornea;
Epidermal Growth Factor*;
Humans*;
Seoul;
Wounds and Injuries*
- From:Journal of the Korean Ophthalmological Society
2001;42(5):745-750
- CountryRepublic of Korea
- Language:Korean
-
Abstract:
PURPOSE: The Influence of topically applied human epidermal growth factor(hEGF) on stromal keratocyte was evaluated in the experimental corneal alkali wounds. METHODS: Corneal alkali wounds were induced by applying a 5.5 mm round filter paper soaked in 1N NaOH onto the center of rabbit cornea for 60 seconds. The eyes were treated topically with 5 micrometer/ml hEGF(DaeWoong Pharma. Co. Ltd. Seoul, Korea) or PBS, 3 times per day. After 3- and 28-days treatment, the corneas were processed for transmission electron microscopy(TEM). RESULTS: After 3-days treatment, the basement membrane integrity was maintained and the stromal keratocyte remained damaged in both groups. However, after 28-days treatment, the basement membrane was disrupted and the keratocytes were activated in both groups. CONCLUSIONS: The findings of present study indicated that the optimal concentration of hEGF which is known to stimulate the epithelial healing was failed to affect the keratocyte activities as long as the basement membrane integrity was maintained.
