Relationship between p38MAPK and GRK2 in spinal cord in development of persistent postoperative pain in rats
10.3760/cma.j.issn.0254-1416.2019.08.015
- VernacularTitle: 大鼠持续性术后痛形成时脊髓p38MAPK与GRK2的关系
- Author:
Xingguo HU
1
;
Le QI
2
;
Gongjian LIU
3
;
Yinming ZENG
3
Author Information
1. Department of Anesthesiology, Taoyuan County People′s Hospital, Hunan Province, Taoyuan 415700, China
2. Jiangsu Province Key Laboratory of Anesthesiology & Jiangsu Province Key Laboratory of Anesthesia and Analgesia Application Technology, Xuzhou Medicine University, Xuzhou 221004, China(is working on the Department of Pain Management, Xuzhou Central Hospital, Xuzhou 221009, China)
3. Jiangsu Province Key Laboratory of Anesthesiology & Jiangsu Province Key Laboratory of Anesthesia and Analgesia Application Technology, Xuzhou Medicine University, Xuzhou 221004, China
- Publication Type:Journal Article
- Keywords:
G protein-coupled receptor kinase 2;
p38 Mitogen-actived protein kinases;
Pain, postoperative;
Spinal cord
- From:
Chinese Journal of Anesthesiology
2019;39(8):957-960
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To evaluate the relationship between p38 mitogen-activated protein kinase (p38MAPK) and G protein-coupled receptor kinase 2 (GRK2) in the development of persistent postoperative pain in rats.
Methods:Pathogen-free healthy male Sprague-Dawley rats, weighing 200-250 g, aged 2 months, were used in this study.Sixty rats in which intrathecal catheters were successfully implanted were divided into 6 groups (n=10 each) using a random number table method: sham operation group (group S), sham operation plus dimethyl sulfoxide (DMSO) group (group D), sham operation plus GRK2 degradation inhibitor MDL28170 group (group M), persistent postoperative pain group (group PPP), persistent postoperative pain plus DMSO group (group PPP+ D) and persistent postoperative pain plus MDL28170 group (group PPP+ M). Persistent postoperative pain was evoked by skin/muscle incision and retraction (SMIR). Immediately after operation and at 1, 2 and 3 days after operation, normal saline 20 μl was intrathecally injected once a day in S and PPP groups, 5% DMSO 10 μl was intrathecally injected once a day in D and PPP+ D groups, and MDL28170 10 μl (50 μg) was intrathecally injected once a day in M and PPP+ M groups.The mechanical paw withdrawal threshold (MWT) was measured at 1 day before operation and 3, 7, 14 and 21 days after operation (T1-4). Four rats in each group were selected after behavioral testing at T3 and sacrificed, and the L4-6 segments of the spinal cord were removed for determination of the expression of phosphorylated p38MAPK (p-p38MAPK) by Western blot.
Results:There was no significant difference in MWT at each time point or expression of p-p38MAPK among group S, group D and group M (P>0.05). Compared with group S, the MWT was significantly decreased, and the expression of p-p38MAPK was up-regulated in PPP, PPP+ D and PPP+ M groups (P<0.05). Compared with group PPP, the MWT was significantly increased, and the expression of p-p38MAPK was down-regulated in group PPP+ M (P<0.05), and no significant change was found in the MWT or expression of p-p38MAPK in group PPP+ D (P>0.05).
Conclusion:Down-regulated expression of spinal GRK2 can promote the activation of p38MAPK in the spinal cord and is involved in the development of persistent postoperative pain in rats.
