COMP-Angiopoietin-1 Promotes Cavernous Angiogenesis in a Type 2 Diabetic Rat Model.
10.3346/jkms.2013.28.5.725
- Author:
Sun Ouck KIM
1
;
Hyun Suk LEE
;
Kyuyoun AHN
;
Kwangsung PARK
Author Information
1. Department of Urology, Chonnam National University Medical School, Gwangju, Korea. uropark@gmail.com
- Publication Type:Original Article ; Research Support, Non-U.S. Gov't
- Keywords:
Erectile Dysfunction;
Rats, Inbred OLETF;
Angiogenesis;
Angiopoietin-1
- MeSH:
Angiopoietin-1/genetics/*metabolism;
Animals;
Antigens, CD31/metabolism;
Blood Glucose/analysis;
Blotting, Western;
Body Weight;
Cartilage Oligomeric Matrix Protein/genetics/*metabolism;
Diabetes Mellitus, Experimental/*pathology;
Immunohistochemistry;
Male;
Neovascularization, Physiologic/*drug effects;
Penis/metabolism/pathology;
Rats;
Rats, Long-Evans;
Recombinant Fusion Proteins/biosynthesis/genetics/*pharmacology;
Vascular Endothelial Growth Factor A/metabolism
- From:Journal of Korean Medical Science
2013;28(5):725-730
- CountryRepublic of Korea
- Language:English
-
Abstract:
Cartilage oligomeric matrix protein-angiopoietin-1 (COMP-Ang1) is an angiogenic factor for vascular angiogenesis. The aim was to investigate the effect of an intracavernosal injection of COMP-Ang1 on cavernosal angiogenesis in a diabetic rat model. Male Otsuka Long-Evans Tokushima Fatty (OLETF) rats made up the experimental group (1 yr old) and Long-Evans Tokushima Otsuka (LETO) rats made up the control group. The experimental group was divided into vehicle only, 10 microg COMP-Ang1, and 20 microg COMP-Ang1. COMP-Ang1 was injected into the corpus cavernosum of the penis. After 4 weeks, the penile tissues of the rats were obtained for immunohistochemistry and Western blot analysis. The immunoreactivity of PECAM-1 and VEGF was increased in the COMP-Ang1 group compared with the vehicle only group. Moreover, the expression of PECAM-1 and VEGF was notably augmented in the 20 microg Comp Ang-1 group. In the immunoblotting study, the expression of PECAM-1 and VEGF protein was significantly less in the OLEFT rats than in the control LETO rats. However, this expression was restored to control level after intracavernosal injection of COMP-Ang1. These results show that an intracavernosal injection of COMP-Ang1 enhances cavernous angiogenesis by structurally reinforcing the cavernosal endothelium.
