Application of NASBA and RPA in detection of pathogenic bacteria
10.3760/cma.j.issn.0254-6450.2019.08.027
- VernacularTitle: NASBA和RPA两种等温扩增技术在病原菌检测中的应用研究
- Author:
Yuan YUE
1
,
2
;
Jianzhong ZHANG
1
;
Maojun ZHANG
1
Author Information
1. Institute for Communicable Disease Prevention and Control, Chinese Center for Disease Control and Prevention, Beijing 102206, China
2. Ningxia Hui Autonomous Region Food Testing and Research Institute, Yinchuan 750001, China
- Publication Type:Review
- Keywords:
Pathogenic bacteria;
Nucleic acid sequence-based amplification;
Recombinase polymerase amplification
- From:
Chinese Journal of Epidemiology
2019;40(8):1018-1022
- CountryChina
- Language:Chinese
-
Abstract:
Nucleic acid sequence-based amplification and recombinase polymerase amplification are the recently developed thermostatic amplification techniques based on PCR. This paper briefly summarizes the principle of reaction, design principle of primer and probe, advantage of these two techniques (simple, accurate, highly sensitive and rapid) and introduces the application of the techniques in the detection of pathogenic bacteria.
