Application of Prkralear-3J mouse as an animal model in the study of congenital microtia
10.3760/cma.j.issn.1009-4598.2019.08.015
- VernacularTitle: Prkra小耳小鼠动物模型在先天性小耳畸形研究中的应用
- Author:
Wei LIU
1
;
Lin LIN
;
Qinghua YANG
;
Bo PAN
;
Leren HE
;
Haiyue JIANG
Author Information
1. First Center of the External Ear Plasty and Reconstruction, Plastic Surgery Hospital, Chinese Academy of Medical Science & Peking Union Medical College, Beijing 100144, China
- Publication Type:Journal Article
- Keywords:
Prkralear-3J mouse;
Microtia;
Animal model;
Prkra gene;
Pact gene;
Alcian blue staining
- From:
Chinese Journal of Plastic Surgery
2019;35(8):804-808
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To explore the feasibility of using Prkralear-3J mouse (also known as: little ears 3 Jackson) as an animal model in the study of congenital microtia.
Methods:Six Prkralear-3J mice of the same litter were introduced from JAX laboratory in the United States. 13 pregnant Prkralear-3J mice were obtained through breeding. A total of 75 fetus tails with gestational age of 14.5 days were obtained, and DNA was extracted and sequenced by Sanger method. Three pregnant mice were normally produced, and the young mice were routinely reared after birth. Three 40-day-old mice with auricle deformity and normal auricle were used respectively to perform skeletal alcian blue staining and comparative analysis.
Results:The auricle of Prkralear-3J homozygous mice was significantly smaller than that of the wild-type mice, and the substructure morphology of the normal mouse auricle was lost. The mutation site was a G-to-A mutation on chromosome 76, 643, 218 bp (GRCm38), which was located after the third exon. The results of alcian blue staining showed that the overall body size of Prkralear-3J homozygous mouse was smaller than that of wild type mouse, and the bone development of limbs was basically normal, but it was thin and short, especially in the hind limbs, the ribs were smaller, the tailbone was shorter, and the skull was slightly smaller.
Conclusions:The Prkralear-3J mouse is a suitable animal model for studying the developmental mechanism of congenital microtia.
