The effect of PM2.5 on oncogene expression in HBE cells
10.3760/cma.j.issn.1001-9391.2019.08.001
- VernacularTitle: PM2.5对HBE细胞癌基因表达水平的影响
- Author:
Kai ZHENG
1
,
2
;
Bingyu WANG
1
,
2
;
Xinyun XU
3
;
Hong GENG
4
;
Dingxin LONG
1
Author Information
1. School of Public Health, South China University, Hengyang 421001, China
2. Institue of Environment and Health, Shenzhen Center for Disease Control and Prevention, Shenzhen 518055, China
3. Institue of Environment and Health, Shenzhen Center for Disease Control and Prevention, Shenzhen 518055, China
4. Institute of Environmental Science, Shanxi University, Taiyuan 030006, China
- Publication Type:Journal Article
- Keywords:
Fine particulate matter (PM2.5);
Haze;
Human bronchial epithelial cells;
Oncogenes;
Gene expression
- From:
Chinese Journal of Industrial Hygiene and Occupational Diseases
2019;37(8):561-566
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To study the effect of particulate matter 2.5 (PM2.5) on oncogene expression in human bronchial epithelial (HBE) cells.
Methods:HBE cells were selected as the study subjects, and PM2.5 treatment group (10 μg/ml and 50 μg/ml) , negative control group and positive control group (10 μmol/L Cr6+) were set. CCK8 assay was used to test the IC50 value of PM2.5. HBE cells were treated with PM2.5 for 24 h at 10 μg/ml and 50 μg/ml, additionally, cells were treated with blank as negative control, 10 μmol/L Cr6+ as a positive control for 24 h. After the treatment, mRNA expression of oncogenes including c-myc, c-fos, k-ras and p53 were detected by fluorescent quantitative RT-PCR, the protein expression of oncogenes were detected with western blot.
Results:The IC50 value of PM2.5 in HBE cells is 70.12 μg/ml. The qRT-PCR data showed that compared with the control group, the expression level of c-myc gene increased by respectively 500.1%、780.7%、305.3% after exposure to 10、50 μg/ml PM2.5 and positive control group; c-fos gene increased respectively 34.0%、76.7%、131.3% after exposure to 10、50 μg/ml PM2.5 and positive control group; k-ras gene increased respectively 50.3%、107.0%、49.7% after exposure to 10、50 μg/ml PM2.5 and positive control group; p53 gene decreased by 28.3%、28.7%、59.7% after exposure to 10、50 μg/ml PM2.5 and positive control group. The western blot results showed that compared with the control group, c-myc protein increased respectively 29.7%、77.3% after exposure to 50 μg/ml PM2.5 and positive control group; c-fos protein increased respectively 200.3%、137.0% after exposure to 50 μg/ml PM2.5 and positive control group; k-ras protein increased respectively 106.3%、130.3%、116.7% after exposure to 10、50 μg/ml PM2.5 and positive control group; p53 protein decreased by 43.7%、53.3%、52.1% after exposure to 10、50 μg/ml PM2.5 and positive control group.
Conclusion:PM2.5 could promote the expression of oncogenes in HBE cells, the carcinogenicity of haze might be related to promotion of oncogenes expression induced by PM2.5.
