Determination of cyclohexanol in urine by headspace solid-phase microextraction coupled with gas chromatography
10.3760/cma.j.issn.1001-9391.2019.07.016
- VernacularTitle: 尿中环己醇测定的顶空-固相微萃取气相色谱法
- Author:
Wanting SU
1
,
2
;
Yong MEI
;
Geng ZHANG
;
Hongmin GAO
;
Menghan NIE
;
Ruodan FANG
Author Information
1. School of Public Health, Wuhan University of Science and Technology, Wuhan 430065, China
2. Hubei Province Key Laboratory of Occupational Hazard Identification and Control, Wuhan University of Science and Technology, Wuhan 430065, China
- Publication Type:Journal Article
- Keywords:
Headspace solid-phase microextraction;
Cyclohexanone;
Cyclohexanol;
Urine;
Chromatography, gas
- From:
Chinese Journal of Industrial Hygiene and Occupational Diseases
2019;37(7):543-546
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To establish a method for determining cyclohexanol in urine by headspace solid-phase microextraction (HS/SPME) coupled with gas chromatography (GC) .
Methods:After the urine sample was hydrolyzed by β-glucuronidase, 2.0 g of NaCl was added, then the analyte in urine was adsorbed by a CAR/PDMS solid phase micro-extraction head in a water bath at 50 ℃ for 20 min. And the extraction head was inserted into the gas chromatograph gasification chamber to desorb, the analyte was detected after separated by the capillary through the flame ionization detector.
Results:The linear range of the method was 0.1-5.0 mg/L with the correlation coefficients (r) 0.999. The average recovery was 84.5%-96.3%, the inter-day precision was 3.81%-6.00%, and the detection limit was 0.03 mg/L.
Conclusion:The method is founded to be low detection limit, simple operation and no need of organic solvent. So it is suitable for the detection of cyclohexanol in urine of occupational exposure to cyclohexanone.
