Protective effect of melatonin against lead-induced neurotoxicity in PC12 cells
10.3760/cma.j.issn.1001-9391.2019.07.001
- VernacularTitle: 褪黑素在铅致神经细胞毒性中的保护作用
- Author:
Yawen LIU
1
;
Fang YE
;
Anli JIANG
;
Jun CHEN
Author Information
1. Department of Occupational and Environmental Health, School of Public Health, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430000, China
- Publication Type:Journal Article
- Keywords:
Lead;
Melatonin;
Neurotoxicity;
Oxidative stress
- From:
Chinese Journal of Industrial Hygiene and Occupational Diseases
2019;37(7):481-486
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To study the protective effect of melatonin (MT) against lead-induced neurotoxicity.
Methods:PC12 cells were divided in four groups (control, 25 μmol/L PbAc, 50 μmol/L MT and 25 μmol/L PbAc+50 μmol/L MT) . After treatment, cell viability was detected by cell counting kit-8 (CCK-8) assay and cytotoxicity LDH assay were used to evaluate cell damage rate. The expression of Cleaved-Caspase-3 and cytochrome C (cyto C) was detected by immunofluorescence (IF) . ROS levels were measured by DCFH method. GSH contents and SOD activities were detected after whole cell protein was extracted.
Results:Compared with control group, 25 μmol/L PbAc caused significantly decreased cell viability, GSH contents and SOD activities, while increased cell damage rate and ROS level significantly (P<0.05) . 25 μmol/L PbAc could also increase the protein level of cleaved-caspase-3 and cyto C in cytoplasm. Compared with 25 μmol/L PbAc group, 50 μmol/L MT+25 μmol/L PbAc caused significantly increased cell viability, GSH contents and SOD activities, while decreased cell damage rate and ROS level significantly (P<0.05) . Compared with 25 μmol/L PbAc group, 50 μmol/L MT+25 μmol/L PbAc decreased the protein level of cleaved-caspase-3 and cyto C.
Conclusion:Melatonin could attenuate PbAc-induced oxidative stress and cell death and promote cell survival.
