Experimental research in athymic nude mice for autologous lymph nodes fragmentary transplantation combined with VEGF-C
10.3760/cma.j.issn.1009-4598.2019.06.017
- VernacularTitle: 联合VEGF-C非血管化淋巴结移植免疫缺陷动物模型的研究
- Author:
Lei CUI
1
;
Na LIN
;
Zheng YANG
;
Chen YANG
;
Wenfang DONG
;
Qinhao GU
;
Yanjun FENG
;
Ye ZHANG
;
Zhoubing WANG
;
Leren HE
Author Information
1. Plastic Surgery Hospital, Chinese Academy of Medical Sciences, Peking Union Medical College, Beijing 100144, China
- Publication Type:Journal Article
- Keywords:
Lymph nodes transplantation;
Vascular endothelial growth factor-C;
Animal model
- From:
Chinese Journal of Plastic Surgery
2019;35(6):607-613
- CountryChina
- Language:Chinese
-
Abstract:
Objective:Autologous lymph nodes fragmentary transplantation combined with vascular endothelial growth factor-C (VEGF-C) on athymic nude mice to explore the association between regeneration of lymphatic vessel and tumor cell migration.
Methods:A total of 45 nude mice were randomly divided into 3 groups: Group A, simple autologous lymph nodes fragmentary transplantation, n=15; Group B, autologous lymph nodes fragmentary transplantation together with VEGF-C, n=15; Group C, without any intervention, n=15. At 1 month, 2 months and 6months after surgeries, the axillary lymph nodes of 5 mice in each group were dynamically examined by in vivo indocyanine green (ICG) fluorescence imaging respectively. The regenerated lymph nodes and relevant skin were evaluated using hematoxylin-eosin (H&E) staining and the skin was quantitatively analyzed via immunofluorescence staining for lymphatic vessel endothelial hyaluronan receptor 1(LYVE-1) as well.
Results:One month after surgery, the right regenerated axillary lymph nodes in group B (5/5) were visible by in vivo ICG fluorescence imaging, whereas the same signals were not detected in group A (0/5). The results were the same at 2 and 6 months after surgery. HE staining showed that the cortical, paracortical, and medullary regions of the right axillary lymph nodes of the experimental group B were clear, and the lymphatic vessel structure was present, accompanied by lymphocyte infiltration. Immunofluorescence staining of the right upper limb showed that the expression of LYVE-1 in the lymphatic endothelium of the B group was significantly higher than that in group A (P<0.001) and the control group (both P<0.001).
Conclusions:Due to the promising consequence of regenerated lymph nodes, the procedure of autologous lymph nodes fragmentary transplantation combined with VEGF-C in athymic nude mice provides a reliable animal model for the next stage research.
