Establishment and application of rapid detection method for influenza virus and subtypes based on flow fluorescence technology
10.3760/cma.j.issn.1003-9279.2019.06.021
- VernacularTitle: 流感病毒基因分型流式荧光法的建立及临床应用研究
- Author:
Dongjie CHEN
1
;
Hongru LI
;
Daxuan WANG
;
Xiaoqin LI
;
Liping HUANG
;
Shanshan HUANG
;
Yina HUANG
;
Yusheng CHEN
Author Information
1. Department of Respiratory Fujian Provincial Hospital, Fuzhou 350001, China
- Publication Type:Journal Article
- Keywords:
Influenza virus;
Subtypes;
Genotyping;
Flow fluorescence technology
- From:
Chinese Journal of Experimental and Clinical Virology
2019;33(6):658-662
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To develop a method for rapid detection of influenza virus and its subtypes based on flow fluorescence technology, and then detect the clinical specimens by our established method .
Methods:We designed degenerate primers and specific probes, synthesized plasmids, used Luminex platform to establish detection method and later detected 430 clinical specimens, and compared the result with those of Fujian Center for Disease Control and prevention.
Results:A method for the simultaneous determination of influenza viruses A, B, C and its subtypes (H116, N19) was established. The time consumption was 3.5 hours, with good specificity, high sensitivity and feasible stability. The detection result of 430 clinical specimens showed high consistency with the result of Fujian Center for Disease Control and Prevention.
Conclusions:We established a method for simultaneous determination of influenza viruses and its subtypes, high sensitivity, specificity and stability.
