Development and application of real-time qPCR assay for detecting Sendai virus gene copy number
10.3760/cma.j.issn.1003-9279.2019.06.016
- VernacularTitle: 仙台病毒颗粒数实时荧光定量PCR检测方法的建立及验证
- Author:
Xiaohuan ZHANG
1
;
Wenhao SU
;
Xiuxiu REN
;
Tingting ZHAO
;
Yinan WANG
;
Jiangbo WEI
Author Information
1. National Vaccine & Serum Institute, Beijing 101111, China
- Publication Type:Journal Article
- Keywords:
Sendai virus;
Real-time fluorescence quantitative PCR;
Recombinant vector vaccine
- From:
Chinese Journal of Experimental and Clinical Virology
2019;33(6):637-640
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To develop a real-time quantitative PCR (qPCR) assay for detecting Sendai virus gene copy number.
Methods:The recombinant plasmid was constructed and a reference used to establish the real-time qPCR method with the TaqMan probe and verified for reproducibility and sensitivity.
Results:The linear range of the developed real-time qPCR assay was 1.024 × 103 ~5 × 1010 copies/μl, with an R2 value of more than 0. 99. The standard recovery of the tested samples was 84.56%~119.48%.
Conclusions:Compared with traditional hernagglutination assay for particle number detection, this method has higher sensitivity, better repeatability and high quantitative accuracy. It can be used for the detection of particle number of vaccine with sendai virus as the carrier, so as to detect the specific activity of vaccine products.
