Generation of human monoclonal antibody Fab fragment to Zika Virus by Phage Display Technology
10.3760/cma.j.issn.1003-9279.2019.06.014
- VernacularTitle: 人源抗寨卡病毒单克隆抗体Fab段基因的获得和表达
- Author:
Runyu YUAN
1
;
Lina SUN
2
;
Juan SU
1
;
Xunmin JI
3
;
Junhe LIANG
4
;
Maoyu CHEN
4
;
Mifang LIANG
2
;
Changwen KE
1
Author Information
1. Guangdong Provincial Center for Disease Control and Prevention, Guangzhou 511430, China
2. National Institute for Viral Disease Control and Prevention, Chinese Center for Disease Control and Prevention, Beijing 102206, China
3. Guangdong Provincial Institute of Biological Products and Materia Medica, Guangzhou 510440, China
4. Jiangmen City Center for Disease Control and Prevention, Jiangmen 529030, China
- Publication Type:Journal Article
- Keywords:
Human genetically engineered antibody;
ZIKV;
Phage display;
Fab fragment
- From:
Chinese Journal of Experimental and Clinical Virology
2019;33(6):626-631
- CountryChina
- Language:Chinese
-
Abstract:
Objective:In this study, phage display technology was used to construct the human anti-Zika virus(ZIKV), phage antibody library and to obtain and express the monoclonal antibody. The aim was to master the preparation and expression of human phage antibody library screening method for highly specific antibodies.
Methods:The whole blood samples of Zika patients were collected and the lymphocytes were isolated. The RT-PCR method was used to amplify the antibody light chain and heavy chain Fab gene from lymphocyte Ig mRNA. The pComb3H system was used to construct the gene with genetic diversity Preparation of human anti-ZIKV phage antibody library. The purified antibody library was screened by using the purified ZIKV and the obtained ZIKV E protein antigen.
Results:The monoclonal antibody Fab fragment gene was successfully obtained for the ZIKV E protein antigen. The gene can be efficiently expressed in Escherichia coli.
Conclusions:According to the sequence analysis, this study showed that the monoclonal antibody was a new human genetically engineered antibody against ZIKV, which laid the foundation for the early diagnosis of ZIKV, and obtain a specific monoclonal antibody to ZIKV for human treatment of ZIKV infection.
