Genetic characteristics of human enterovirus A group 71 type in Xining City in 2017
10.3760/cma.j.issn.1003-9279.2019.06.009
- VernacularTitle: 西宁市2017年人肠道病毒A组71型基因特征
- Author:
Qing SONG
1
;
Liping REN
2
;
Tianjiao JI
3
;
Dongmei YAN
3
Author Information
1. The First People′s Hospital of Xining, Xining 810000, China
2. Liaoning Center for Disease Control and Prevention, Shenyang 110005, China
3. National Institute for Viral Disease Control and Prevention, Chinese Center for Disease Control and Prevention, Beijing 100052, China
- Publication Type:Journal Article
- Keywords:
Hand, foot and mouth disease;
Enterovirus-A71;
Subgenotype C4a
- From:
Chinese Journal of Experimental and Clinical Virology
2019;33(6):603-605
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To investigate the genetic characteristics of enterovirus A group 71 type (EV-A71) and etiological features of hand, foot and mouth disease (HFMD) in Xining city in 2017.
Methods:The pharyngeal swab specimens were collected from HFMD patients, and detected by real-time reverse transcription-polymerase chain reaction (RT-PCR). For EV-A71 positive samples, virus isolation was performed. Then RNA of EV-A71 strains was extracted, and then VP1 coding region was amplified by RT-PCR. The phylogenetic tree was constructed by comparing with other genotypes and sub-genotypes strains of EV-A71.
Results:Total 57 strains of EV-A71 were isolated in Xining city in 2017, which all belonged to genotype C4a, and could be divided into two different lineages by phylogenetic analysis. The epidemic strains of EV-A71 in Xining City was closely related to other provinces of China in 2017.
Conclusions:C4a was the dominant genotype of EV-A71 in Xining city in 2017, and no other genotype was detected. In addition, EV-A71 isolated from Xining city was co-evolved with EV-A71 in other provinces of China.
