Analysis of the pathogen spectrum of severe hand, foot and mouth disease and genetic characteristics of enterovirus A71 in Xianyang, 2018
10.3760/cma.j.issn.1003-9279.2019.06.007
- VernacularTitle: 咸阳市2018年手足口病重症病例病原谱及肠道病毒A71型基因特征分析
- Author:
Junjun ZHANG
1
;
Bin CHEN
;
Yingying WANG
;
Nan YANG
;
Lijuan WANG
;
Xuan LI
Author Information
1. Xianyang City Center for Disease Control and Prevention, Xianyang 712000, China
- Publication Type:Journal Article
- Keywords:
Hand, foot and mouth disease;
Severe;
EV-A71;
Genetic characteristic analysis
- From:
Chinese Journal of Experimental and Clinical Virology
2019;33(6):593-597
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To understand the pathogen spectrum of severe hand, foot and mouth disease (HFMD), and analyze the genetic characteristics of enterovirus A71(EV-A71) in Xianyang in 2018.
Methods:Totally 67 specimens of severe cases of HFMD were collected. Enteroviruses associated with HFMD were detected by real-time PCR and the genotypes of enteroviruses were identified by VP4 region of enteroviruses. The nucleotide and amino acid sequences of VP1 region of EV-A71 were analyzed.
Results:A total of 30 samples were positive for enterovirus among samples from 67 severe cases with HFMD, including 9 cases of EV-A71, 11 cases of coxsackievirus A6 (CV-A6), 5 cases of coxsackievirus A16 (CV-A16), 2 cases of coxsackievirus A10 (CV-A10) and 2 cases of coxsackievirus A4 (CV-A4). The nucleotide and amino acid homologies of EV-A71 among 4 strains reached 96.7%-99.9% and 99.3%-100% respectively. The 4 strains of EV-A71 belonged to C4a subtypes by phylogenetic analysis. The six amino acid composite model was KADSTV in 4 strains of EV-A71. The EF region and GH region in antigenic determinants of 4 strains of EV-A71 kept consistent with representative reference strains, however, the EV-A71 SZK222 and SZK497 strains developed mutation at site 93 (I93V) of BC loop region.
Conclusions:EV-A71 and CV-A6 are major agents of severe HFMD in Xianyang in 2018. The genotype of EV-A71 belonged to C4a subtype and the VP1 gene did not show more mutations.
