Molecular epidemiological characteristics of respiratory syncytial virus in pediatric patients in Gansu province from 2012 to 2017
10.3760/cma.j.issn.1003-9279.2019.06.006
- VernacularTitle: 2012—2017年甘肃省感染儿童中呼吸道合胞病毒的分子流行特征
- Author:
Ruijuan QIAO
1
;
Jianhua CHEN
1
;
Haizhuo WU
1
;
Yan ZHANG
2
;
Hui ZHANG
1
;
Peng WANG
1
;
Ruxue ZHANG
3
;
Yuning LI
4
;
Qiang GAO
5
;
Kemin WEI
6
;
Deshan YU
1
Author Information
1. Gansu Center for Disease Control and Prevention, Lanzhou 730000, China
2. National Institute for Viral Disease Control and Prevention, Chinese Center for Disease Control and Prevention, Beijing 102206, China
3. Baiyin Center for Disease Control and Prevention, Baiyin 730900, China
4. Department of Pediatrics, the first Hospital of Lanzhou University, Lanzhou 730000, China
5. Longnan Center for Disease Control and Prevention, Longnan 746000, China
6. Tianshui Center for Disease Control and Prevention, Tianshui 741000, China
- Publication Type:Journal Article
- Keywords:
Respiratory syncytial virus;
G protein;
Genetic characteristics;
Molecular epidemiology
- From:
Chinese Journal of Experimental and Clinical Virology
2019;33(6):586-592
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To analyze the genotypes, amino acid vatiations and molecular epidemiological characteristics of respiratory syncytial virus (RSV) infection in pediatric patients in Gansu province for the future research.
Methods:A total of 4 556 respiratory tract specimens were colleted from pediatric patients under 10 years of age in five cities in Gansu from 2012 to 2017. These specimens were tested for RSV and its subtypes.The coding region of the RSV G gene was amplified using reverse transcription-polymerase chain reaction (RT-PCR) and sequenced for RSV positive specimens. Sequences were edited using DNA Star software. Sequence alignment and phylogenetic trees were built by MEGA 6.0 software.
Results:Out of 4 556 specimens, 1 135 (24.91%) were positive for RSV, totally 216 G protein sequences were obtained. RSV A isolates were clustered into three genotypes: NA1、NA3 and ON1. The nucleotides and amino-acid homology was 84.9%-100% and 77.3%-100%, respectively. The nucleotides and amino-acid homology between this study and prototype long strain was 81.2%-83.3% and 74.1%-88.0%. RSV B isolates were clustered into only BA9 one genotypes. The nucleotides and amino-acid homology was 97.7%-100% and 95.8%-100%, respectively. The nucleotides and amino-acid homology between this study and prototype CH18537 strain was 84.9%-85.7% and 77.9%-80.1%.
Conclusions:The genetic characteristics and the amino-acid changes were analyzed systematically using data of RSV G gene collected from 2012 to 2017 in Gansu province in this study. These data were used for analyses of the etiology, control and prevention of RSV infection.
