Up-regulation of peroxiredoxin 3 by high-risk human papillomavirus in cervical cancer cells
10.3760/cma.j.issn.1003-9279.2019.06.004
- VernacularTitle: 宫颈癌细胞过氧化物还原酶3的表达与高危型人乳头瘤病毒的相关性分析
- Author:
Xiaojuan SUN
1
;
Xiaoyan LI
2
;
Jingmin LI
2
;
Houli LIU
3
;
Ranran LIU
1
;
Lianqin LI
1
;
Shaobing ZHAN
4
Author Information
1. Department of Reproductive Medicine, Yantai Affiliated Hospital of Binzhou Medical University, Yantai 264100, China
2. School of Basic Medical Sciences, Binzhou Medical University, Yantai 264003, China
3. Department of Laboratory Medicine, Yantai Affiliated Hospital of Binzhou Medical University, Yantai 264100 China
4. National Institute for Viral Disease Control and Prevention, Chinese Center for Disease Control and Prevention, Beijing 100052, China
- Publication Type:Journal Article
- Keywords:
Cervical cancer;
Human papillomavirus;
Oxidative stress;
Peroxiredoxin
- From:
Chinese Journal of Experimental and Clinical Virology
2019;33(6):576-579
- CountryChina
- Language:Chinese
-
Abstract:
Objective:The present study was conducted to investigate the response of peroxiredoxin 3 (PRX3) to oxidative stress induced by high-risk human papillomavirus (HPV).
Methods:Sixty patients with cervical cancer were included and sixty patients with hysteromyoma were assigned as controls. Serum PRX3 was detected by enzyme-linked immunosorbent assay. The expression of PRX3 and oncoprotein E6 of HPV16 or HPV18 was examined in cervical cancer tissues by immunohistochemistry and in cervical cancer cells by Western blotting respectively.
Results:Patients with invasive squamous cervical cancer showed higher level of serum PRX3 than control subjects with hysteromyoma. PRX3 expression was up-regulated and was positively associated with that of E6 of HPV16 or HPV18 in cervical cancer tissues. The correlation was confirmed in HPV-containing cervical cancer cell lines including CaSki, and HeLa.
Conclusions:Our result indicated a positive response of PRX3 to HPV-induced oxidative stress. Serum PRX3 might be a potential indicator of active amplification of high-risk HPV in cervical cancer cells.
