Analysis on the epidemiology and etiologic characteristics of first imported severe fever with thrombocytopenia syndrome case in Shenzhen
10.3760/cma.j.issn.1003-9279.2019.06.003
- VernacularTitle: 深圳市首例发热伴血小板减少综合征病例的流行病学和病原学特征研究
- Author:
Fan YANG
1
;
Yalan HUANG
;
Xiaomin ZHANG
;
Yaqing HE
;
Linghong XIONG
;
Yue LI
;
Renli ZHANG
Author Information
1. Institute of Pathogenic Biology, Shenzhen Center for Disease Control and Prevention, Shenzhen 518055, China
- Publication Type:Journal Article
- Keywords:
Severe fever with thrombocytopenia syndrome;
Bunyavirus;
Epidemiology;
Etiology
- From:
Chinese Journal of Experimental and Clinical Virology
2019;33(6):570-575
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To study the epidemiology and the etiology characteristics of first imported severe fever with thrombocytopenia syndrome (SFTS) case reported in Shenzhen city in 2017.
Methods:Data on descriptive epidemiology was collected to study the characteristics to the epidemic. The serum sample collected from the suspect SFTS case was detected for IgM, IgG by ELISA and severe fever with thrombocytopenia syndrome bunyavirus (SFTSV) nucleic acid by real-time RT-PCR. The samples were further inoculated in Vero cell for virus isolation. The partial fragements of L and S gene were amplified by RT-PCR and sequenced to construct homology comparison and phylogenetic tree with the strains isolated from other areas.
Results:The case was laboratory confirmed imported SFTS case in Shenzhen on May 2017. IgM antibody and RNA of SFTSV were detected in the serum sample. SFTSV named GDSZ01/2017/China was successfully isolated from the serum sample. The high nucleotide homology of L and S genome segments were found at 95.3%-98.2% and 93.8%-98.8% with other representative strains from the popular provinces, respectively. The phylogenetic tree indicated that GDSZ01 was most close to SDTA_3 strain, next to strains in Hubei procince. The isolated SFTSV belonged to genotype C3 with HB29, HB154.
Conclusions:The virological, serological and molecular features showed that the imported case of SFTS in 2017 was caused by SFTSV C3 genotype.
