Discovery of the expression of HK2 gene in clear cell carcinoma of the kidney and its clinical significance based on database analysis
10.3760/cma.j.issn.1673-4181.2019.05.007
- VernacularTitle: 基于数据库分析HK2基因在肾透明细胞癌中的表达及临床意义
- Author:
Wei LI
1
;
Dongsheng ZHU
Author Information
1. Department of Urology, the Secondary Hospital of Tianjin Medical University, Tianjin Institute of Urology, Tianjin 300211, China
- Publication Type:Journal Article
- Keywords:
Renal clear cell carcinoma;
Hexokinase 2;
Bioinformatics
- From:
International Journal of Biomedical Engineering
2019;42(5):398-403
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To analyze the association between hexokinase 2 (HK2) gene expression and clinical pathological characteristics in renal clear cell carcinoma using database and bioinformatics methods.
Methods:Oncomine database was used to analyze the most significant differentially expressed genes between renal clear cell carcinoma and non-kidney tissue. The expression levels of mRNA and protein were detected by GPEIA and The Human Protein Atlas database. Correlation of HK2 expression level between clinicopathological features and prognosis of renal clear cell carcinoma was analyzed using LinkedOmics and KM-plotter databases, respectively. The STRING database was used to predict the potential protein interaction mechanism.
Results:The most significant difference protein in renal clear cell carcinoma, i.e. HK2, was found, which was highly expressed in renal clear cell carcinoma tissues, and positively correlated with pathological stage, T stage and N stage of renal cell carcinoma (all P<0.05). The overall survival rate of the renal clear cell carcinoma patients with high expression of HK2 was significantly lower than that of the patients with low expression (P<0.05).
Conclusions:High expression of HK2 gene may be associated with pathological staging, high T stage, high N stage, and poor prognosis of renal clear cell carcinoma.
