Clinical study of brush liquid-based cytology combined with automatic immunohistochemistry in the classification and diagnosis of lung cancer
10.3760/cma.j.issn.0253-3766.2019.05.002
- VernacularTitle: 纤维支气管镜刷检液基薄片细胞学联合免疫细胞化学在肺癌病理分型诊断中的临床价值
- Author:
Linlin ZHAO
1
;
Yue SUN
;
Cong WANG
;
Jian CAO
;
Huiqin GUO
;
Huan ZHAO
;
Zhihui ZHANG
Author Information
1. Cytopathology Section, Department of Pathology, National Cancer Center/National Clinical Research Center for Cancer/Cancer Hospital, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing 100021, China
- Publication Type:Journal Article
- Keywords:
Lung neoplasms;
Fiberoptic bronchoscopy;
Liquid-based cytology;
Immunocytochemistry;
Pathological classification
- From:
Chinese Journal of Oncology
2019;41(5):326-330
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To explore the feasibility of bronchoscopic brushing liquid-based slide cytology combined with automatic immunocytochemistry (ICC) for pathological typing of lung cancer.
Methods:A liquid-based thin-prep was prepared from 171 bronchoscopic brushing specimens of patients with pulmonary lesions. ICC was detected by automatic immunohistochemistry instrument while cytomorphological diagnosis was made. The results were compared with those of histopathological diagnosis.
Results:Among 171 patients, 130 (76.0%) could be classified by cell morphology alone, including 31 squamous cell carcinomas, 44 adenocarcinomas and 55 small cell carcinomas; 162 (94.7%) could be classified by cell morphology combined with ICC, including 38 squamous cell carcinomas, 61 adenocarcinomas and 63 small cell carcinomas (P<0.001). According to the gold standard of histopathological diagnosis, the coincidence rate of cytomorphology combined with ICC was higher than that of cell morphology alone. The coincidence rate of squamous cell carcinoma was increased from 85.2% to 97.1% (P=0.093), adenocarcinoma from 92.5% to 98.0% (P<0.001), and small cell carcinoma from 96.1% to 98.3% (P=0.465).
Conclusion:The combination of liquid-based thin-prep cytology and automatic immunohistochemistry can effectively improve the accuracy of pathological typing of brushing specimens under fiberoptic bronchoscopy, and provide more objective diagnostic results for clinical treatment.
