Concentration of hepatitis A virus from mimicked water samples by membrane filtration method
10.3760/cma.j.issn.1003-9279.2019.05.017
- VernacularTitle: 滤膜吸附洗脱法富集模拟水样中甲型肝炎病毒的研究
- Author:
Lin ZHAO
1
;
Xinying WANG
;
Feng QIU
;
Jingyuan CAO
;
Shengli BI
Author Information
1. NHC Key Laboratory of Medical Virology and Viral Diseases, National Insitute of Viral Disease Control and Prevention, Chinese Center for Disease Control and Prevention, Beijing 102206, China
- Publication Type:Journal Article
- Keywords:
Hepatitis A virus;
Concentration;
Mimicked water sample;
Real-time PCR
- From:
Chinese Journal of Experimental and Clinical Virology
2019;33(5):522-525
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To optimize the membrane filtration method for hepatitis A virus concentration from mimicked water samples.
Methods:Mimicked water samples containing HAV particles were prepared and concentrated by positively charged membrane and negatively charged membrane respectively. Then different method including direct lysis, shaker, vortex and ultrasonication were used to elute HAV followed by the quantification of HAV by Taqman Real-time RT-PCR. The data were analyzed by professional statistical software.
Results:In the present study, when mimicked water samples contained 300 TCID50 of HAV, there was no significant difference between the concentration effects by negatively charged membrane and positively charged membrane (P=0.825>0.05). However, when HAV in mimic water samples was up to 1500 TCID50 and 7500 TCID50, the recycle efficiency by negatively charged membrane was higher than that by positively charged membrane (P<0.01). Additionally, this study found that HAV recycle ratio could be up to (68.17±16.79)% when direct lysis was used for viral elution, therefore direct lysis was proved much better than shaker, vortex and ultrasonication. They were significantly different (P<0.0001).
Conclusions:Elution process played the key role in HAV concentration when membrane filtration method was used. Direct lysis was proved much better than other method and it was the most efficient way in HAV recycle from mimicked water samples.
