The role of lectin-like Ox-LDL receptor-1 on endothelial cell injury caused by human cytomegalovirus infection
10.3760/cma.j.issn.1003-9279.2019.05.012
- VernacularTitle: 凝集素样氧化型低密度脂蛋白受体-1在人巨细胞病毒感染致内皮细胞损伤中的作用
- Author:
Jingjing CHE
1
;
Huiqi WANG
2
;
Yitong DU
1
;
Xiaojiao XU
1
;
Li YI
1
;
Ruijin WANG
1
;
Jun CHEN
1
;
Lei LIU
3
;
Dexin WANG
1
;
Houzhen TUO
1
Author Information
1. Department of Neurology, Beijing Friendship Hospital, Capital Medical University, Beijing 100150, China
2. Neurological Disease Rehabilitation Center, Beijing Rehabilitation Hospital, Capital Medical University, Beijing 100041, China
3. Department of Neurology, Tongren Hospital, Capital Medical University, Beijing 100730, China
- Publication Type:Journal Article
- Keywords:
Atherosclerosis;
Human Cytomegalovirus;
Lectin-like Ox-LDL receptor-1;
Oxidative stress;
Vascular cellular adhesion molecule-1;
Umbilical vein endothelial cells
- From:
Chinese Journal of Experimental and Clinical Virology
2019;33(5):500-503
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To investigate the role of lectin-like Ox-LDL receptor-1( LOX-1) in the activation and oxidative stress of cultured human umbilical vein endothelial cell(HUVEC) after human cytomegalovirus(HCMV) infection.
Methods:HUVEC were divided into four groups: HCMV, Control, Carrageenan, and HCMV+ Carrageenan. After HCMV AD169 infection, the supernatant of the culture was extracted, and cells were lysed. The levels of LOX-1 mRNA, intercellular adhesion molecule-1(ICAM-1) mRNA and vascular cellular adhesion molecule-1(VCAM-1) in HUVEC were measured by real-time PCR. And the content of nitrogen monoxidum(NO) of the supernatant was detected by nitrate reductase method accordingly.
Results:24 h after infection, the mRNA expression of LOX-1, ICAM-1 and VCAM-1 in HUVEC of HCMV infected group increased obviously compared to control, and NO quantity increased accordingly. The mRNA expression of LOX-1, ICAM-1 and VCAM-1 and the quantity of NO decreased after adding the LOX-1 inhibitor carrageenan. There was significant difference between groups(P<0.05).
Conclusions:HCMV may increase the mRNA expression of ICAM-1 and VCAM-1 and quantity of NO by upregulating the mRNA expresion of LOX-1, which may contribute to the formation of a therosclerosis(AS).
