Genetic characteristics of VP1 region of enterovirus Coxsackievirus A16 in Xining city from 2017 to 2018
10.3760/cma.j.issn.1003-9279.2019.05.008
- VernacularTitle: 西宁市2017—2018年柯萨奇A16型肠道病毒VPl基因分子特征分析
- Author:
Haijie GENG
1
;
Liping REN
2
;
Yongjian FENG
1
;
Huirong ZHANG
1
;
Xiaohong WANG
1
;
Chonghai LI
3
;
Xiaotong WANG
3
;
Lixia FAN
3
Author Information
1. Health Inspection and Testing Center, Xining Center for Disease Control and Prevention
2. Institute of Immunization planning, Liaoning Center for Disease Control and Prevention
3. Health Inspection and Testing Center, Qinghai Center for Disease Control and Prevention, Xining 810007, China
- Publication Type:Journal Article
- Keywords:
Hand, foot and mouth disease;
Coxsackievirus A16;
Genetic characteristics
- From:
Chinese Journal of Experimental and Clinical Virology
2019;33(5):482-484
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To investigate the genetic characteristics of VP1 coding region of enterovirus Coxsackievirus A16(CV-A16) and etiological features of hand, foot and mouth disease (HFMD) in 2017 in Xining city.
Methods:The pharyngeal swab specimens were collected from HFMD patients, and detected by real-time reverse transcription-polymerase chain reaction (RT-PCR). For CV-A16 positive samples, virus isolation was performed. Then RNA was extracted, and then VP1 coding region was amplified by RT-PCR. The phylogenetic tree was constructed by comparing with other genotypes and sub-genotypes strains of EV-A71.
Results:It was shown that 70 strains of CV-A16 were isolated from 2017 to 2018 in Xining city. In 2017, 10 strains were isolated and divided into two different lineages by phylogenetic analysis, 3 strains of B1a and 7 stains of B1b. In 2018, 60 stains were isolated, which were all belong to B1b.
Conclusions:B1a and B1b of CV-A16 are prevalent in Xining city from 2017 to 2018, in which B1b is the prominent isolates.
