Pyroptosis induced by different Enteroviruses infection in SH-SY5Y cell
10.3760/cma.j.issn.1003-9279.2019.05.002
- VernacularTitle: 不同肠道病毒诱导SH-SY5Y细胞焦亡研究
- Author:
Qiao QIAO
1
;
Tao WU
;
Xiaojuan ZHU
;
Ying CHI
;
Yiyue GE
;
Huan FAN
;
Yuhua QI
;
Xiling GUO
;
Lunbiao CUI
Author Information
1. Jiangsu Provincial Center for Disease Control and Prevention, NHC Key Laboratory of Enteric Pathogenic Microbiology, Nanjing 210009, China
- Publication Type:Journal Article
- Keywords:
Enterovirus;
Caspase-1;
Pyroptosis
- From:
Chinese Journal of Experimental and Clinical Virology
2019;33(5):454-457
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To investigate the pyroptosis induced by different enteroviruses in human neuroblastoma cells SH-SY5Y and the differences among them.
Methods:SH-SY5Y cells were infected with nine strains of enterovirus respectively, including enterovirus A71 (EV-A71), Coxsackievirus A (CA), Coxsackievirus B (CB), Echovirus (Echo). The cellular morphology of infected and control groups were observed and activity of Caspase-1 of infected and control groups were detected by flow cytometry at 48 h post infection.
Results:The activity of Caspase-1 induced by EV-A71 was higher than control (P<0.001), and the activity of Caspase-1 induced by EV-A71 isolated from severe case was significantly higher than that induced by EV-A71 isolated from common case (P<0.001). The activity of Caspase-1 induced by CA was at a lower level. The activity of Caspase-1 induced by CB and Echo were both significantly higher than that induced by EV-A71 and control (P<0.001). Cytopathic effects (CPE) were found to be related with the activity of Caspase-1.
Conclusions:EV-A71, CB and Echo all could induce pyroptosis mediated by Caspase-1 in SH-SY5Y cells, but the ability of CA to induce pyroptosis was at a lower level, which may provide evidences for further study on mechanism of neuropathy caused by enterovirus.
