Mechanism of mesenchymal stem cells-derived extracellular matrix influences on ADSCs: changing microenvironment
10.3760/cma.j.issn.1009-4598.2019.04.007
- VernacularTitle: 间充质干细胞细胞外基质改变脂肪来源干细胞微环境影响其生物学行为的机制分析
- Author:
Zichao LI
1
;
Xiaowei YI
2
;
Chenggang YI
3
Author Information
1. Department of Burns and Cutaneous Surgery, First Affiliated Hospital of Air Force Military Medical University, Xijing Hospital , Xi′an 710032 , China
2. Department of Epidemiology and Health Statistics, West China School of Public Health, Sichuan University, Chengdu 610041, China
3. Department of Plastic Surgery, First Affiliated Hospital of Air Force Military Medical University , Xijing Hospital , Xi′an 710032 , China
- Publication Type:Journal Article
- Keywords:
Adipose-derived stem cells;
Mesenchymal stem cells;
Cell culture;
Collagen-encoding gene;
Enrichment analysis
- From:
Chinese Journal of Plastic Surgery
2019;35(4):361-366
- CountryChina
- Language:Chinese
-
Abstract:
Objective:This study aims to explore the potential related gene and mechanism of mesenchymal stem cells (MSCs)-derived extracellular matrix (ECM) promoting the proliferation and differentiation of adipose-derived stem cell (ADSCs), through regulating extracellular microenvironment.
Methods:Twelve transcriptomes were analysed using Gene Expression Omnibus (GEO) database, and divided into four groups: (1) ADSCs cultured in Tissue Culture Polystyrene (TCPS) medium with normal human dermal fibroblasts (NHDF)-derived ECM, (2) ADSCs cultured in TCPS medium with bone marrow mesenchymal stem cells (BMSCs)-derived ECM.(3) ADSCs cultured in TCPS medium with ADSCs-derived ECM.(4) ADSCs cultured in TCPS medium as the control group. Kyoto Encyclopedia of Genes and Genomes (KEGG), Gene Ontology (GO), and Gene Set Enrichment Analysis (GSEA). Software R, Perl and Cytoscape software were used for differential expression analysis and data visualization.
Results:Each MSC produced specific extracellular matrix. However, the ADSCs cultured with additive ECM expressed partially different genes and proteins, e. g. The collagen family genes. Furthermore, 3 important molecules: COL4A1, COL11A1, COL15A1 were detected by constructing the interaction relationship between the shared genes and the functional groups. They may affect the proliferation and differentiation of ADSCs by changing microenvironment. The low expression of above molecules, may be related to biological processes and signaling pathways, such as cell repair, nucleotide metabolism, DNA replication, cell cycle, etc.
Conclusions:The gene expression of collagen encoding were down-regulated, when ADSCs cultured in the medium with additive ECM derived by MSCs. This may significantly affect the proliferation and differentiation of ADSCs through various pathways.
