The anti-photoaging effects of human umbilical cord mesenchymal stem cells derived extracellular vesicles on dermal fibroblasts
10.3760/cma.j.issn.1009-4598.2019.04.004
- VernacularTitle: 人脐带间充质干细胞来源细胞外囊泡抗皮肤成纤维细胞光老化作用研究
- Author:
Mingwu DENG
1
;
Dong LI
;
Ziyou YU
;
Xiangsheng WANG
;
Wei LI
;
Wenjie ZHANG
Author Information
1. Department of Plastic and Reconstructive Surgery, Shanghai 9th People′s Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai Key Laboratory of Tissue Engineering, National Tissue Engineering Center of China, Shanghai 200011, China
- Publication Type:Journal Article
- Keywords:
Skin photoaging;
Extracellular vesicles;
Antioxidation
- From:
Chinese Journal of Plastic Surgery
2019;35(4):341-346
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To investigate the anti-photoaging effects of human umbilical cord mesenchymal stem cells derived extracellular vesicles (hucMSC-EVs) on dermal fibroblasts.
Methods:Human fibroblasts were pretreated with hucMSC of different concentrations for 24 hours before ultraviolet B (UVB) irradiation. Immediately post irradiation, the intracellular reactive oxygen species (ROS) were analyzed using reactive oxygen detection kit. After 72 hours irradiation, cell proliferation was assessed using CCK-8 assay, and the percentage of senescent cells was evaluated by β-galactosidase (SA-β-gal) staining.
Results:The UVB irradiation increased intracellular ROS level, decreased the cell proliferation from 100% to(77.33±2.89)%, and increased the percentage of SA-β-gal positive senescent cells from (6.70±0.46)% to (17.67±1.53)%. The hucMSC-EVs pretreatment decreased the intracellular ROS level, stimulated cell proliferation from (77.33±2.89)% to (90.67±2.52)% and( 96.00±5.57)% , and decreased the positive rate of SA-β-gal positive cells from (17.67±1.53)% to (8.38±0.56)% and (7.07±1.10)%.
Conclusions:The hucMSC-EVs preconditioning may protect dermal fibroblasts from UVB induced photoaging, by reducing intracellular ROS.
