Etiologic spectrum of hand, foot and mouth disease and the genetic characteristics of the Coxsackievirus A10 and A6 in Qingdao in 2014
10.3760/cma.j.issn.1003-9279.2019.04.010
- VernacularTitle: 青岛市2014年手足口病病原谱及柯萨奇病毒A10、A6型VP1区基因特征
- Author:
Xiaoyan SHI
1
;
Yanhui ZHANG
2
;
Dan ZHAO
1
;
Zhilei SU
1
;
Qing CHAI
1
;
Jinling GONG
1
;
Zhaoguo WANG
1
Author Information
1. Qingdao Municipal Center for Disease Control and Prevention, Qingdao Institute of Preventive Medicine, Qingdao 266033, China
2. Qingdao Women and Children Hospital, Qingdao 266034, China
- Publication Type:Journal Article
- Keywords:
Hand, foot and mouth disease;
Coxsackievirus A10;
Coxsackievirus A6;
Phylogenetic analysis
- From:
Chinese Journal of Experimental and Clinical Virology
2019;33(4):380-384
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To investigate the etiology spectrum of hand foot and mouth disease (HFMD) and to analyze the molecular characteristics of Coxsackievirus A10 and A6 in Qingdao in 2014.
Methods:Throat swabs of HFMD cases were tested for total enteroviruses (EVs), EV-A71, CV-A16, CV-A10 and CV-A6 by multiplex real time RT-PCR. Other EV serotypes were identified through the sequences of partial VP1 gene. The full-length of VP1 gene of CV-A10 and CV-A6 were amplified and sequenced. The sequences were phylogenetically analyzed using MEGA 5.0 software package.
Results:A total of 1727 HFMD patients were detected in 2014 and 11serotypes of enteroviruses were identified. EV-A71(38.0%, 410/1078), CV-A16(28.8%, 311/1078), CV-A10(14.1%, 152/1078)and CV-A6(3.2%, 34/1078)were the most dominant pathogen in 2014 in Qingdao. Proportions of CV-A10 in enterovirus infected children varied dramatically in different ages(χ2=15.19, P=0.001), showing a downtrend with age. Proportion of CV-A10 in inpatients was much higher than that in outpatients(χ2=49.1, P <0.001), while 60% of those inpatients showed nervous system damage symptoms, with pathological reflex or disappearance of physiological reflex. Phylogenetic analysis of complete VP1 sequences showed that all CV-A10 strains identied in this study belonged to genotype C, 71.7% of which located in branch C5; all CV-A6 strains belonged to genotype D while 83.3% of which located in branch D5.
Conclusions:EV-A71, CV-A16, CV-A10 and CV-A6 were the prevalent pathogens of HFMD in Qingdao in 2014. CV-A10 was more frequently detected in lower age group with HFMD, which can cause damage to nervous system. Strains of branch C5 in genotype C were the dominant strains of CV-A10 circulated in Qingdao in 2014 while strains of branch D5 in genotype D were the major strains of CV-A6.
