Genetic characterization analysis of 2B genotype rubella virus strains in Liaoning province
10.3760/cma.j.issn.1003-9279.2019.04.004
- VernacularTitle: 辽宁省首次分离2B基因型风疹病毒基因特征分析
- Author:
Yan WANG
1
;
Shuang HAO
1
;
Wensi WANG
1
;
Yinan HAN
2
;
Zhi ZHANG
3
;
Xing FANG
1
;
Wenqing YAO
1
Author Information
1. The Institute for Immunization Programme, Liaoning Provincial Center for Disease Control and Prevention, Shenyang 110005 China
2. The Institute for Immunization Programme, Dalian Center for Disease Control and Prevention 116021, China
3. The Institute for Immunization Programme, Shenyang Center for Disease Control and Prevention, Shenyang 110031, China
- Publication Type:Journal Article
- Keywords:
Rubella Virus;
2B genotype;
Phylogenetic analysis
- From:
Chinese Journal of Experimental and Clinical Virology
2019;33(4):354-357
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To analyze the genetic characterization of 2B genotype rubella virus strains first isolated in Liaoning province.
Methods:Vero/Slam cells were used to isolate rubella viruses from throat swabs. Fragments of the E1 gene (739 nucleotides) were amplified by reverse transcription-polymerase chain reaction (RT-PCR) and the PCR products were sequenced and analyzed. The phylogenetic trees were constructed with rubella 2B genotypes reference strains.
Results:RVi/Liaoning.CHN/13.18/1[2B] was isolateded from the case which had a history of international travel and probably imported from abroad. RVi/Liaoning.CHN/13.18/1[2B] was in the same 1ineage with RVs/Edinburgh.GBR/6.12[2B] and RVs/Kawasaki.JPN/17.11/1[2B], but far from the epidemic strains isolated in China in recent years. RVi/Liaoning.CHN/14.18/1[2B] belonged to the same lineage with rubella virus 2B genotype strains isolated in China from 2014 to 2015, and had the highest homology with RVi/Chouzhou.Anhui.CHN/17.15/2[2B] and RVi/Shaoyang.Zhejiang. CHN/13.13[2B].
Conclusions:It was the first time to isolate the 2B genotype rubella virus in Liaoning province and the strain belonged to different 1ineage.
