Determination of seven urinary metabolites of benzene, toluene and xylene by ultra-high performance liquid chromatography-triple quadrupole mass spectrometry
10.3760/cma.j.issn.1001-9391.2019.04.017
- VernacularTitle: 尿中苯和甲苯及二甲苯七种生物标志物测定的超高效液相色谱-三重四极杆质谱法
- Author:
He QIAO
1
,
2
;
Xiaodong LIU
1
;
Xiangjuan MENG
1
;
Jue LI
1
;
Dongsheng NIU
1
;
Xiaowen DING
1
;
Jun NIE
3
Author Information
1. Beijing Institute of Occupational Medicine for Chemical Industry, Beijing Chemical Industry Group Co., Ltd., Beijing 100093, China
2. Beijing University of Chemical Technology, Beijing 100029, China
3. Beijing University of Chemical Technology, Beijing 100029, China
- Publication Type:Journal Article
- Keywords:
Urinary metabolites of benzene, toluene and xylene;
Urine;
Chromatography, liquid;
Triple quadrupole mass spectrometry
- From:
Chinese Journal of Industrial Hygiene and Occupational Diseases
2019;37(4):303-307
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To develop a method using ultra-high performance liquid chromatography-triple quadrupole mass spectrometry to determine the urinary metabolites of benzene, toluene and xylene. The selected metabolites are S-phenylmercapturic acid (S-PMA) , trans, trans-muconic acid (t, t-MA) , 8-hydroxy-2 deoxyguanosine (8-OHdG) , hippuric acid (HA) , 2-methylhippuric acid (2-MHA) , 3-methylhippuric acid (3-MHA) and 4-methylhippuric acid (4-MHA) .
Methods:The urine sample was pretreated using methanol to precipitate the proteins. HSS T3 chromatographic column was used to separate the metabolites. The mass spectrometric acquisition was carried out using multiple reaction monitoring (MRM) after ionization with ESI source. External standard method was used for quantification.
Results:All the standard curves showed good linear relation, and r of the seven metabolites was all above 0.999. The detection limits and quantitative limits of the seven metabolites were 0.01-500 ng/ml and 0.02-1 000 ng/ml (based on the actual dilution ratio) , respectively. The average spiked recoveries of four loadings ranged from 85.8% to 109.9%. The intra-day and inter-day precisions were 0.2%-4.5% and 0.6%-9.5%, respectively. The samples can be kept for at least 14 days at both 4 ℃ and -20 ℃.
Conclusion:This method is simple, rapid and highly sensitive with low cost, and its accuracy, precision and stability can meet the daily test requirements. It can be applied for the determination of urinary S-PMA, t, t-MA, 8-OHdG, HA, 2-MHA, 3-MHA and 4-MHA for the occupational population exposed to benzene, toluene and xylene.