Clinical implications and prognostic value of TP53 gene mutation and deletion in patients with myelodysplastic syndromes
10.3760/cma.j.issn.0253-2727.2019.03.010
- VernacularTitle: 伴TP53基因异常骨髓增生异常综合征患者的临床特征及预后研究
- Author:
Huijun HUANG
1
;
Zhongxun SHI
;
Bing LI
;
Tiejun QIN
;
Zefeng XU
;
Hongli ZHANG
;
Liwei FANG
;
Naibo HU
;
Lijuan PAN
;
Shiqiang QU
;
Dan LIU
;
Yanan CAI
;
Yudi ZHANG
;
Zhijian XIAO
Author Information
1. Institute of Hematology and Blood Diseases Hospital CAMS & PUMC, The State Key Laboratory of Experimental Hematology, Tianjin 300020, China
- Publication Type:Journal Article
- Keywords:
Myelodysplastic syndromes;
TP53 gene;
Mutation;
Deletion;
Prognosis
- From:
Chinese Journal of Hematology
2019;40(3):215-221
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To explore the clinical implications and prognostic value of TP53 gene mutation and deletion in patients with myelodysplastic syndromes (MDS) .
Methods:112-gene targeted sequencing and interphase fluorescence in situ hybridization (FISH) were used to detect TP53 mutation and deletion in 584 patients with newly diagnosed primary MDS who were admitted from October 2009 to December 2017. The association of TP53 mutation and deletion with several clinical features and their prognostic significance were analyzed.
Results:Alterations in TP53 were found in 42 (7.2%) cases. Of these, 31 (5.3%) cases showed TP53 mutation only, 8 (1.4%) cases in TP53 deletion only, 3 (0.5%) cases harboring both mutation and deletion. A total of 37 mutations were detected in 34 patients, most of them (94.6%) were located in the DNA binding domain (exon5-8) , the remaining 2 were located in exon 10 and splice site respectively. Patients with TP53 alterations harbored significantly more mutations than whom without alterations (z=-2.418, P=0.016) . The median age of patients with TP53 alterations was higher than their counterparts[60 (21-78) years old vs 52 (14-83) years old, z=-2.188, P=0.029]. TP53 alterations correlated with complex karyotype and International prognostic scoring system intermediate-2/high significantly (P<0.001) . Median overall survival of patients with TP53 alterations was shorter than the others[13 (95%CI 7.57-18.43) months vs not reached, χ2=12.342, P<0.001], while the significance was lost during complex karyotype adjusted analysis in multivariable model.
Conclusion:TP53 mutation was more common than deletion in MDS patients. The majority of mutations were located in the DNA binding domain. TP53 alterations were strongly associated with complex karyotype and always coexisted with other gene mutations. TP53 alteration was no longer an independent prognostic factor when complex karyotype were occurred in MDS.
