miRNA-96-5p inhibits the proliferation and migration of gastric cancer cells by targeting FoxQ1
10.3760/cma.j.issn.0253-3766.2019.03.008
- VernacularTitle: miRNA－96－5p靶向调控叉头框蛋白Q1的表达抑制胃癌细胞增殖侵袭和上皮间质转化
- Author:
Xinyi YANG
1
;
Ning LI
2
;
Wenying DENG
2
;
Yijie MA
2
;
Xueling HAN
2
;
Zhongyu ZHANG
2
;
Jinling XIE
3
;
Suxia LUO
4
Author Information
1. Department of Pharmacy, Affiliated Cancer Hospital of Zhengzhou University, Zhengzhou 450008, China
2. Department of Gastroenterology, Affiliated Cancer Hospital of Zhengzhou University, Zhengzhou 450008, China
3. Department of Oncology, the First Affiliated Hospital of Xinxiang Medical College, Xinxiang 453100, China
4. Department of Oncology, Affiliated Cancer Hospital of Zhengzhou University, Zhengzhou 450008, China
- Publication Type:Journal Article
- Keywords:
Gastric neoplasms;
MicroRNA;
Forkhead box Q1;
Proliferation;
Invasion;
Epithelial-mesenchymal transition (EMT)
- From:
Chinese Journal of Oncology
2019;41(3):193-199
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To investigate the role of microRNA-96-5p in the proliferation and invasion of gastric cancer cells and its molecular mechanism.
Methods:From June 2015 to January 2017, 53 resected specimens were collected. The transcriptional levels of microRNA-96-5p and forkhead box Q1 (FoxQ1) in gastric cancer tissues and the matched para-cancerous tissues were quantified by quantitative real-time PCR (qRT-PCR). The expression of FoxQ1 protein was also detected by immunohistochemistry (IHC). The relationship between microRNA-96-5p expression and the clinicopathological features of gastric cancer and its correlation with FoxQ1 expression were analyzed. The expressions of miRNA-96-5p in gastric cancer tissue and adjacent normal tissue were detected by qRT-PCR. miRNA-96-5p mimics was transfected to BGC-823 gastric cancer cells. The effects of miRNA-96-5p on cell proliferation and invasion were detected by cell counting kit-8 (CCK-8) assay and Transwell assay, respectively. The protein expressions of FoxQ1, E-cadherin and vimentin were determined by western blot. The relationship between FoxQ1 and miRNA-96-5p expressed in BGC-823 cells was detected by dual-luciferase reporter assay.
Results:The median expression of miRNA-96-5p in gastric cancer tissue was 1.05, significantly lower than 3.23 of para-cancerous tissues (P<0.05). The positive rate of FoxQ1 expression in gastric cancer tissue was 71.7%, significantly higher than 28.3% of para-cancerous tissues (P<0.05). The expression of FoxQ1 was negatively corelated with the level of miRNA-96-5p (r=-0.613, P=0.006). The expression of miRNA-96-5p in gastric cancer cell BGC-823 was significantly decreased compared with normal gastric epithelial cell (0.96±0.08 vs 2.84±0.15, P<0.05). The results of CCK-8 assay and Transwell assay showed that overexpression of miRNA-96-5p significantly reduced the proliferation and invasion abilities of gastric cancer cells (P<0.05). Overexpression of miRNA-96-5p decreased the protein level of FoxQ1. Moreover, it upregulated the expression of E-cadherin and downregulated the expression of vimentin. The result of dual-luciferase-3′-UTR reporter assay confirmed that miRNA-96-5p binds to the 3′UTR of FoxQ1.
Conclusion:miRNA-96-5p may suppress the proliferation, migration and epithelial-mesenchymal transition (EMT) of gastric cancer cell by down-regulation of FoxQ1.
