Antimicrobial resistance and molecular epidemiology of foodborne Yersinia enterocolitica in Pudong New District, Shanghai
10.3760/cma.j.issn.0254-6450.2019.03.019
- VernacularTitle: 上海市浦东新区食源性小肠结肠炎耶尔森菌耐药及分子流行病学特征
- Author:
Wenqing WANG
1
,
2
;
Qiqi CUI
1
,
2
;
Xiao WANG
1
,
2
;
Yongqi ZHANG
1
,
2
;
Caiyun LI
1
,
2
;
Jinghua SU
1
,
2
;
Bing ZHAO
1
,
2
;
Hong HUANG
1
,
2
;
Linying ZHU
1
,
2
;
Xuebin XU
3
;
Lipeng HAO
4
Author Information
1. Department of Microbiology of Pudong New District Center for Disease Control and Prevention, Shanghai 200136, China
2. Pudong Institute of Preventive Medicine, Fudan University, Shanghai 200136, China
3. Institute for Microbiology of Shanghai Municipal Center for Disease Control and Prevention, Shanghai 200336, China
4. Pudong Institute of Preventive Medicine, Fudan University, Shanghai 200136, China
- Publication Type:Journal Article
- Keywords:
Yersinia enterocolitica;
Raw food;
Antimicrobial resistance;
Molecular type
- From:Chinese Journal of Epidemiology
2019;40(3):354-359
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To investigate the antimicrobial resistance and molecular epidemiology of foodborne Yersinia (Y.) enterocolitica in Pudong New District of Shanghai.
Methods:Four kinds of raw food samples were collected in retail circulation sites in Pudong from 2012 to 2016. Cold enrichment method was used to isolate Y. enterocolitica and further detection of biotype, serotype, virulent genes, antimicrobial susceptibility of the isolates and pulsed field gel electrophoresis (PFGE) were conducted.
Results:A total of 3 900 raw food samples were collected during this period, including poultry product (n=590), livestock product (n=1 074), aquatic product (n=1 488), vegetable (n=748), in which 111 (2.8%) were contaminated by Y. enterocolitica. The detection rates of Y. enterocolitica in poultry product samples (5.3%, 31/590) and livestock product samples (4.5%, 48/1 074) were higher than those in aquatic product samples (1.6%, 24/1 488) and vegetable samples (1.1%, 8/748). The predominant biotype was 1A (95.5%) and predominant serotype was O∶8 (42.3%). All the strains lacked ail, ystA, yadA and virF genes, which encoded pathogenic Y. enterocolitica. Seventy six (68.5%) strains harbored ystB gene, in which 35 (31.5%) belonged to 1A/O∶8/ystB pattern. Most strains were resistant to ampicillin (74.8%) and amoxicillin/clavulanic acid (70.3%), and non-sensitive rate to Cefoxitin was over 50.0%. No third generation cephalosporin or fluoroquinolone resistant strains were detected, but 38.7% (43/111) strains were multidrug resistant (MDR). Serotype O∶8 and O∶5 strains had 44 and 18 PFGE patterns, respectively.
Conclusions:The main foodborne exposure sources of Y. enterocolitica in raw food were poultry and livestock products in Pudong New District. 1A/O∶8/ystB was the predominant pattern with potential pathogenicity despite lacks of typical pathogenic virulent genes. The antimicrobial resistant rates of Y. enterocolitica were at a low level, but MDR strains still existed. Molecular types of the isolates showed highly genetic diversity.
