Construction of influenza B virus vero cell adapted strain by genetic reassortment
10.3760/cma.j.issn.1003-9279.2019.03.019
- VernacularTitle: 遗传重配制备B型流感病毒Vero细胞适应毒株
- Author:
Yina CUN
1
;
Shaohui SONG
1
;
Min SU
2
;
Ze LIU
1
;
Yanan WU
1
;
Jingxia GAO
1
;
Lei MA
1
;
Guoyang LIAO
1
;
Weidong LI
1
Author Information
1. Institute of Medical Biology, Chnese Academey of Medical Sciences and Peking Union Medical University, Kunming 650118, China
2. The First People′s Hospital of Yunnan Provience, Kunming 650032, China
- Publication Type:Journal Article
- Keywords:
Vero cell;
Influenza B virus;
Genetic reassortment
- From:
Chinese Journal of Experimental and Clinical Virology
2019;33(3):319-322
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To construct influenza B virus Vero cell adapted strain by genetic recombination technology by using the influenza B virus Vero cell adapted strain as the parent strain.
Methods:The chick embryo and Vero cell were co-infected with influenza virus Vero cell adapted strain B/Malaysia/2506/2004 Va (Bv) and the vaccine strain B/massachusetts/2/2012 (BX-51B) recommended by WHO. The reassortants were screened with the anti-Bv serum. Plaque-purified reassortants were used to screen for Vero cell-adapted influenza B virus strains containing the surface antigen of the epidemic strain.
Results:A Vero cell-adapted influenza B virus strain was obtained with successive passage in Vero cells. The hemagglutination inhibition test and the one-way immunogold agar diffusion test both showed that the reassortant virus was homologous to NYMC BX-51B, and sequence analysis result showed that the reassortment virus has the same HA and NA gene with the vaccine strain.
Conclusion:B/Malaysia/2506/2004Va (Bv) can be used as a parent strain to prepare Vero cell vaccine against influenza B virus.
