Pathogen spectrum of viral encephalitis in children living in Hebei province, China from May to December 2017
10.3760/cma.j.issn.1003-9279.2019.03.011
- VernacularTitle: 2017年5月至12月河北省儿童病毒性脑炎病原学研究
- Author:
Tao FAN
1
;
Yanjie HAN
2
;
Ruiqin ZHANG
2
;
Panhui YU
1
;
Li ZHAO
3
;
Juju QI
3
;
Xinna LI
4
;
Ruihuan WANG
5
;
Yishuo SUN
2
;
Jian ZHAO
1
;
Chuanze HU
3
;
Ji WANG
4
;
Suzhen SUN
1
;
Xuejun MA
4
Author Information
1. Children, s Hospital of Heibei Province Affliated to Hebei Medical University, Shijiazhuang 050031, China
2. Blood Transfusion Department of the Fourth Hospital of Shijiazhuang, Shijiazuang 050011, China
3. Hebei Medical University, Shijiazhuang 050017, China
4. NHC Key Laboratory of Medical Virology and Viral Diseases, National Institute for Viral Disease Control and Prevention Chinese Center for Diseae Corotrol and Prevention, Beijing 102206, China
5. University of South China, Hengyang 421001, China
- Publication Type:Journal Article
- Keywords:
Viral encephalitis;
Pathogen;
Automatic laboratory station;
Real-time fluorescent quantitative PCR
- From:
Chinese Journal of Experimental and Clinical Virology
2019;33(3):275-279
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To investigate the pathogenic characteristics of viral encephalitis in children living in Hebei province.
Methods:We randomly collected cerebrospinal fluid specimens from a total of 399 children diagnosed with viral encephalitis in Hebei Children′s Hospital from May to December 2017. Real-time fluorescence quantitative PCR and Sanger sequencing were used to detect viral nucleic acids in cerebrospinal fluid by an automatic laboratory station. Statistical analysis was performed on the experimental data using SPSS 21.0 software and the clinical data were analyzed. Comparison of infection rates of EV encephalitis in different months, using line × column chi-square test. The MRI and EEG positive rates of different viral encephalitis and viral encephalitis patients not infected with the virus were analyzed by Fisher′s exact probability test. The positive rate of infection with different viruses and non-virus agents was analyzed by Fisher′s exact probability test.
Results:The result showed that 80 of 399 samples were positive, and the positive rate was 20.05%. It included 22 cases of enterovirus, 4 cases of influenza A virus, 3 cases of mumps virus, 2 cases of herpes simplex virus type 1, 1 case of herpes simplex virus type 2, 4 cases of EB virus, 7 cases of cytomegalovirus, 7 cases of herpes zoster virus, 8 cases of adenovirus, 14 cases of human herpesvirus type 6. Eight cases had combined viral infection. Eight cases had concurrent infections: 3 cases had enterovirus and herpesvirus type 6 concurrent infection, 1 case had enterovirus and Japanese encephalitis virus concurrent infection and 1 case had herpes simplex virus type 2 and adenovirus, 1 case had influenza A virus herpesvirus type 6, 1 case had mumps virus and herpesvirus type 6, 1 case had mumps virus and herpesvirus type 6, 1 case had herpes simplex virus type 1 and herpes zoster virus concurrent infections. Children with EV viral encephalitis in Hebei Province were highly prevalent in May and June (P=0.016). HHV6 virus encephalitis was more susceptible to infection than non-HHV6 virus (P=0.016); The rate of MRI positive findings in patients with different viral encephalitis was not statistically significant (P>0.05). The result of EEG of different viral encephalitis were P>0.05, which was not statistically significant.
Conclusions:EV was the most common pathogen of children with viral encephalitis in Hebei province. Encephalitis caused by influenza A virus cannot be ignored in clinical practice.
