Etiological characteristics of an imported Chikungunya fever epidemic in Fujian province in 2018
10.3760/cma.j.issn.1003-9279.2019.03.006
- VernacularTitle: 2018年福建省输入性基孔肯雅热的病原学特征
- Author:
Jinzhang WANG
1
;
Naipeng KAN
1
;
Yongjun ZHANG
1
;
Libin YOU
1
;
Yuping WANG
2
;
Yanqin DENG
1
;
Kuicheng ZHENG
1
;
Yuwei WENG
1
Author Information
1. Fujian Provincial Center for Disease Control and Prevention, Fujian Provincial Key Laboratory of Zoonosis Research, Fuzhou 350001, China
2. Fujian International Travel Healthcare Center, Fuzhou 350001, China
- Publication Type:Journal Article
- Keywords:
Chikungunya virus;
Molecular epidemiology;
E1 gene;
Sequence analysis
- From:
Chinese Journal of Experimental and Clinical Virology
2019;33(3):253-256
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To study the etiological characteristics of an imported Chikungunya fever (CHIK) epidemic in Fujian province in 2018.
Methods:Serum samples collected at different days after the onset of the two CHIK cases were detected by real-time RT-PCR and ELISA. Structural protein E1 gene was amplified by RT-PCR and sequenced for nucleotide characteristics analysis and phylogenetic tree analysis.
Results:RNA of Chikungunya virus (CHIKV) was detected in the 4 serum samples collected on the first 5 days of the disease, and the earliest IgM antibodies were detected in specimens on the 5th day of the disease, however, IgG antibodies were only detected in specimen on 10th day. Compared with the S27-African prototype strain, 12 mutant points were found in the amino acids of E1 genes in this study. The E1 genes of the two CHIK cases were exactly the same, and they were closest to the evolutionary relationship with the strain isolated in the Philippines in 2014. Their genotype was Asian genotype.
Conclusions:This epidemic was confirmed to have been imported from the Philippines after the infection with the Asian genotype CHIKV, which suggests that Fujian province should strengthen the monitoring of persons entering from the CHIK epidemic area, so as to prevent imported cases from causing local outbreaks.
