Changes of endogenous small interfering RNA in H1-HeLa cells induced by human rhinovirus 16 infection
10.3760/cma.j.issn.1003-9279.2019.03.005
- VernacularTitle: 16型鼻病毒感染引起H1-HeLa细胞的内源性siRNA变化
- Author:
Juan SONG
1
,
2
;
Mi LIU
;
Hengshun CHENG
;
Bingtian SHI
;
Wenjun WANG
;
Zhiqiang XIA
;
Qinqin SONG
;
Tianyu CAO
;
Dong XIA
;
Jun HAN
Author Information
1. State Key Laboratory of Infectious Disease Prevention and Control
2. National Institute for Viral Disease Control and Prevention, Chinese Center for Disease Control and Prevention, Collaborative Innovation Center for Diagnosis and Tereatment of Infections Disdese, Beijing 100052, China
- Publication Type:Journal Article
- Keywords:
siRNA;
Human rhinovirus 16;
High-throughput sequencing
- From:
Chinese Journal of Experimental and Clinical Virology
2019;33(3):248-252
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To observe the changes of endogenous small interfering RNA (siRNA) in H1-Hela cells infected with human rhinovirus 16 (HRV 16).
Methods:To determine whether HRV16 infection induced the changes of siRNA, H1-HeLa cells were infected with HRV16 for 12 h, 24 h and 36 h, siRNAs were detected by high-throughput sequencing, second-generation sequencing) and qRT-PCR.
Results:The result showed that siRNA was generated differently at different time points post-infection, among which novel_sir907 and novel_sir1950 decreased at three time points. Further validation by qRT-PCR showed that novel_sir907 decreased at 12 h, 24 h and 36 h post-infection compared with the cell control, but novel_sir1950 increased at 12 h then decreased at 24 h and 36 h.
Conclusions:HRV16 infection induces changes endogenous siRNAs.
