A preliminary study on thapsigargin promoting coxsackievirus B3 replication through activating transcription factor 6 pathway
10.3760/cma.j.issn.1003-9279.2019.03.004
- VernacularTitle: 毒胡萝卜素通过ATF6途径促进柯萨奇病毒B3复制的初步研究
- Author:
Qinqin SONG
1
;
Xiaonuan LUO
;
Juan SONG
;
Dong XIA
;
Bingtian SHI
;
Mi LIU
;
Zhiqiang XIA
;
Wenjun WANG
;
Tianyu CAO
;
Hengshun CHENG
;
Jun HAN
Author Information
1. National Institute for Viral Disease Control and Prevention, Chinese Center for Disease Control and Prevention, State Key Laboratory of Infectious Disease Prevention and Control, Collaborative Innovation Center for Diagnosis and Treatment of Infectious Diseases, Beijing 102206, China
- Publication Type:Journal Article
- Keywords:
Coxsachieviuus B3;
Thapsigargin;
Endoplasmic reticulum stress;
Plaque;
ATF6 pathway
- From:
Chinese Journal of Experimental and Clinical Virology
2019;33(3):244-247
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To investigate the effect of thapsigargin (TG) which can induce endoplasmic reticulum stress (ERS) on the replication of coxsackievirus B 3 (CV-B3).
Methods:After 10 MOI CV-B3 infected HeLa cells were exposed 0.25 μmol/L TG for 3 h, 6 h and 9 h, virus RNA of HeLa cells were extracted and viral replication was evaluated by real time PCR. After 0.25 μmol/L、0.08 μmol/L and 0.025 μmol/L TG exposed, the plaque of CV-B3 was used to confirm further replication of CV-B3. To verify TG induced ERS through three signal pathway, one of among PERK, ATF6 and IRE1 inhibitors GSK2656157, AEBSF and STF-083010, and 0.25 μmol/L TG were used in HeLa cells infected with 10 MOI CV-B3, replication of CV-B3 was evaluated by qRT-PCR.
Results:The stimulation of TG did not induce increase of virus replication after post-infection 3 h. However, TG induced replication of virus to increase 2.5 times after post-infection 6 h and 158.6 times after post-infection 9 h. And, the area of viral plaque was significantly increased. ATF6 inhibitors AEBSF significantly inhibited promotion of virus replication from TG.
Conclusions:TG can promote the replication of CV-B3 through ATF6 signal pathway.
