Analysis of the potential role of SET in chromium-induced malignant transformation cells based on quantitative proteomics

Zhihong Chen; Nuanyuan Luo; Xiaohu Ren; Shuqi Wang; Chao Huang; Yungang Liu; Jianjun Liu

Return

Analysis of the potential role of SET in chromium-induced malignant transformation cells based on quantitative proteomics

VernacularTitle: 基于定量蛋白质组学技术的铬致细胞恶化转化中SET的潜在作用分析
Author: Zhihong CHEN ^{1
,

2} ; Nuanyuan LUO ^{1
,

2} ; Xiaohu REN ³ ; Shuqi WANG ³ ; Chao HUANG ³ ; Yungang LIU ¹ ; Jianjun LIU ^{1
,

2}
Author Information

1. Southern Medical University, School of Public Health, Guangzhou 510515, China
2. Key Laboratory of Modern Toxicology of Shenzhen, Shenzhen Center for Disease Control and Prevention, Shenzhen 518055, China
3. Key Laboratory of Modern Toxicology of Shenzhen, Shenzhen Center for Disease Control and Prevention, Shenzhen 518055, China
Publication Type:Journal Article
Keywords: Proteomics; Hexavalent chromium; 16HBE cells; Patient SE translocation
From: Chinese Journal of Industrial Hygiene and Occupational Diseases 2019;37(3):169-173
CountryChina
Language:Chinese
Abstract: Objective:To investigate alteration of proteins profile in malignant transformation bronchial epithelial cells(16HBE-T) induced by hexavalent chromium[(Cr(VI))] and analyze the expression level of SET protein, then to provide some new insights for the carcinogenesis mechanism of Cr(VI).
Methods:Total protein was extracted from 16HBE cells and was alkylated and desalinated before digested into peptides. The products were labeled with Tandem Mass Tag (TMT) and identified using LC-ESI-MS/MS.
Results:A total of 3 517 proteins were found, expression differences greater than 1.5 or less 0.67 times were to found have 185 and 201 proteins, respectively. Gene enrichment analysis revealed that differential proteins were mainly involved in autophagy, DNA damage repair, RNA processing and other biological processes. Western blot results showed the expression level of SET was significantly increased while downregulated in histone H3K18/27 acetylation and p53 protein.
Conclusion:Proteins involved in multiple biological processes altered in 16HBE-T cells and regulation mode of SET inhibiting histone H3K18/27 acetylation regulating transcriptional activity of p53 may paly an important role in Cr(VI)-association carcinogenesis.