Effects of urea on proliferation and apoptosis of infantile hemangioma derived endothelial cells
10.3760/cma.j.issn.1009-4598.2019.02.017
- VernacularTitle: 尿素对婴幼儿血管瘤内皮细胞增殖和凋亡影响的体外研究
- Author:
Cuiling LANG
1
;
Hongzhao LEI
;
Bin SUN
;
Yubin GONG
;
Miaomiao LI
;
Hongyu ZHANG
;
Changxian DONG
Author Information
1. Department of Hemangioma Surgery of Henan Province People′s Hospital and Henan University, Zhengzhou 450003, China (is working on the Department of Translation Medical Center of Huaihe Hospital, Kaifeng 475000, China)
- Publication Type:Journal Article
- Keywords:
Urea;
Human hemangioma endothelial cells;
Human normal vascular endothelial cells;
Cell activity;
Apoptosis
- From:
Chinese Journal of Plastic Surgery
2019;35(2):189-194
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To observe the effects of different concentrations and doses of urea on the proliferation and apoptosis of human hemangioma endothelial cells, in order to provide evidence for the further mechanism study of urea in the treatment of hemangioma.
Methods:Human hemangioma endothelial cells (HemECs) and normal endothelial cells (VE) were cultured in vitro. Cell viability was detected by CCK-8 after invention with different concentrations(40%, 50%, 60%, 70%) and doses(3, 6, 9 μl/ml) of urea. The apoptosis of HemECs was detected by flow cytometry dual-dye and propidium lodide single dye.
Results:The viability of HemECs was significantly lower than that of VE under different concentrations and doses of urea (P<0.05). The inhibition rate of 40% urea on HemECs increased with the increase of urea dose (P<0.05), and the inhibition effect was most obvious at 4 h and 12 h. The apoptosis of HemECs increased in a time and dosage dependent manner with the treatment of 40% urea. High dose(9 μl/ml)of 40% urea significantly promoted the apoptosis of HemECs(P<0.05).
Conclusions:Low dose of 40% urea significantly inhibited the proliferation of HemECs, and had no significant effect on VE. However, high doses of urea promoted apoptosis of HemECs.
