Expression and receptor binding characteristics of porcine P6 genotype rotavirus GST-VP8*-Z84 protein
10.3760/cma.j.issn.1003-9279.2019.02.022
- VernacularTitle: 猪源P[6]型轮状病毒GST-VP8*-Z84蛋白的表达及受体结合特征
- Author:
Mengxuan WANG
1
;
Luyao WANG
2
;
Dandi LI
1
;
Jiayan ZHANG
3
;
Jihong HU
2
;
Zhaojun DUAN
1
Author Information
1. National Institute for Viral Disease Control and Prevention, Chinese Center for Diseases Control and Prevention, Beijing 102206, China
2. School of Public Health, University of Chinese Medicine, Lanzhou 730000, China
3. Department of Food and Chemical Engineering, Lushan College of Guangxi University of Science and Technology, Liuzhou 545000, China
- Publication Type:Journal Article
- Keywords:
Rotavirus;
GST-VP8*-Z84 protein;
Histo-blood group antigens;
Receptor binding
- From:
Chinese Journal of Experimental and Clinical Virology
2019;33(2):217-220
- CountryChina
- Language:Chinese
-
Abstract:
Objective:The gene engineering technique was used to express the P[6] genotype rotavirus (rotaviruses, RVs) GST-VP8*-Z84 protein from pigs, and the binding characteristics of the protein to oligosaccharide and salivary receptor were studied.
Methods:The GST-VP8*-Z84 protein was purified by GST Escherichia coli expression system and affinity chromatography using porcine P[6]. Enzyme-linked immunosorbent assay (ELISA) saliva binding test and oligosaccharide binding test were used to analyze the binding characteristics of the genotype to receptors.
Results:Porcine P[6] GST-VP8*-Z84 protein bound well to mucin core 2.
Conclusions:The potential receptor of P[6] RV may be the core of mucin, which may provide the experimental basis and theoretical basis for the mechanism of rotavirus and receptor interaction and the development of RV vaccine and highly effective therapeutic drugs.
