Risk factor and hepatitis B virus makers during the first trimester in villi tissues of HBsAg-positive pregnant women
10.3760/cma.j.issn.1003-9279.2019.02.015
- VernacularTitle: HBsAg阳性孕妇早期流产绒毛组织中HBV标志物检测及风险因素研究
- Author:
Tingting PENG
1
;
Miao WANG
2
;
Feng CHEN
2
;
Hong YU
2
;
Min YANG
2
;
Huaxin HUANG
2
;
Lu CUI
2
;
Qiu′e CAI
2
;
Qiang WANG
2
;
Jizhou GOU
2
;
Yan SUN
2
;
Chuming CHEN
2
;
Yingxia LIU
2
Author Information
1. University of South China, Hengyang 421000, China
2. State Key Discipline of Infectious Diseases, Department of Infectious Diseases, Shenzhen Third People′s Hospital, Shenzhen 518112, China
- Publication Type:Journal Article
- Keywords:
Hepatitis B virus;
Villi tissues;
qRT-PCR;
Immunohistochemistry;
Intrauterine infection
- From:
Chinese Journal of Experimental and Clinical Virology
2019;33(2):182-186
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To determine whether intrauterine infection with hepatitis B virus (HBV) occurs in early pregnancy and to characterize associated virulence factors.
Methods:Villi tissues and blood samples of 45 HBV surface antigen (HBsAg)-positive pregnant women were collected during the first trimester and HBV DNA loads were quantified by quantitative reverse transcription polymerase chain reaction (qRT-PCR). The expression of GCM1, HBsAg and hepatitis B core antigen (HBcAg) in villi tissues were detected by immunohistochemical method.
Results:Data from qRT-PCR showed that HBV DNA was detected in 14 of 45 villi tissues (positive rate of 31.11%), and 24 of 45 blood samples (positive rate of 53.33%), further statistical analysis showed that the positive rates of HBV DNA between blood samples and villi tissues were not significantly different (χ2=4.555, P=0.054). Among them, 12 samples were consistently positive between the villi and blood specimens, and HBsAg, HBeAg, HBeAb, HBV DNA from peripheral blood in these pregnant women were significantly higher than those of the other women (P value was 0.007, 0.004, 0.000, and 0.000 respectively). The multivariate logistic regression analysis showed that blood HBV DNA greater than 106 IU/ml was independently associated with HBV DNA positive in villi, and the HBsAg, HBeAg, villi tissues HBV DNA positive rates of these pregnant women were significantly higher than those of the other pregnant women (all P value were 0.000). Immunohistochemistry results showed that all 45 cases were positive for GCM1 expression in the cell nucleus. Nine cases also had HBsAg expression in the cytoplasm. Only one case was found to express HBV core antigen (HBcAg) in the nucleus.
Conclusions:HBV DNA and HBsAg can be detected from villi tissues harvested during the first trimester in HBsAg-positive pregnant women, and the results suggest an early occurrence of intrauterine infection of fetuses with high HBV levels.
