Genetic characteristic analysis of the VP1 gene of echovirus 30 isolated from viral meningitis cases in Yunnan province, 2010-2013
10.3760/cma.j.issn.1003-9279.2019.02.009
- VernacularTitle: 云南省2010—2013年病毒性脑膜炎病例中埃可病毒30型的VP1基因特征分析
- Author:
Lifang HE
1
;
Hui LI
;
Kai LI
;
Zhixian ZHAO
;
Zhengrong DING
;
Bingjun TIAN
Author Information
1. Yunnan Center for Disease Control and Prevention, Kunming 650022, China
- Publication Type:Journal Article
- Keywords:
Echovirus 30;
VP1 gene;
Genetic characteristics analysis
- From:
Chinese Journal of Experimental and Clinical Virology
2019;33(2):158-162
- CountryChina
- Language:Chinese
-
Abstract:
Objective:In this study we analyzed the genetic characteristics of echovirus 30 (E-30) VP1 gene sequences from Yunnan province isolated from viral meningitis (VM) cases in 2010-2013.
Methods:RT-PCR and VP1 gene sequencing were done for 9 E-30 strains isolated from VM cases in 2010-2013. VP1 gene sequences of E-30 reference strains were downloaded from the GenBank and their nucleotide (nt) and amino acid (aa) diversities were calculated by MEGA 5.1 software, the phylogenetic tree was constructed and the genetic characteristics and molecular epidemiology were analyzed.
Results:In 2010-2013, 9 strains of E-30 viruses were detected from 79 VM cases caused by echoviruses, accounting for 11.39%(9/79), the overall positive rate was 1.63%(9/553). Phylogenetic analysis revealed that E-30 strains can be divided into four genotypes (genotype A, B, C and D), and genotype D can be further divided into seven sub-genotypes. Nine Yunnan VM isolates were distributed in D7 sub-genotype, and can be further clustered into 3 branches: 5 strains isolated in 2010 were clustered in branch 1, it is evident that these viruses were responsible for an aseptic meningitis outbreak in Kunming in that year; one 2011 isolate, together with 2013 isolate and one isolate from healthy children in 2010 were clustered in branch 2, these two branches were Yunnan special branches, and two 2011 isolates had the highest homology with 2003 VM outbreaks′ strains isolated from Shandong, Jiangsu, and Zhejiang, showing that these strains may have the same evolutionary sources.
Conclusions:Nine Yunnan VM isolates were distributed in D7 sub-genotype, and these strains have different evolutionary sources, showing that at different times E-30 viruses in the same sub-genotypes branch might prevail in different areas.
