Genomic sequences analysis of human Norovirus GII.4 variant JN010 in Jinan
10.3760/cma.j.issn.1003-9279.2019.02.008
- VernacularTitle: 诺如病毒GII.4型济南JN010株基因组特征分析
- Author:
Huailong ZHAO
1
;
Hengyun GUAN
;
Guoliang YANG
;
Hong ZHAO
;
Yan LYU
;
Ying HAN
;
Chunrong WANG
;
Lanzheng LIU
Author Information
1. Jinan Municipal Center for Disease Control and Prevention, Jinan 250021, China
- Publication Type:Journal Article
- Keywords:
Norovirus;
GII.4 genotype;
Genome;
Phylogenetic analysis
- From:
Chinese Journal of Experimental and Clinical Virology
2019;33(2):152-157
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To analyse the genomic characteristics of Novirus(NoV) GII.4 genotype JN010 strain isolated form Jinan in 2017.
Methods:Seven pairs of primers were designed and used to amplify the JN010 genome. Sequence analyses, alignment and phylogenetic trees of ORF1 and ORF2 genes were performed using the software Lasergene7.1 and MEGA5.2. At the same time the major protein VP1 amino acid mutations were analyzed.
Results:The 7 516 bp complete genome sequence of JN010 strain was obtained, the most mutation sites were located in P2 subdomain of VP1. Two substitutions I293N and H373N of VP1 were locate neighboring epitope A, and R297H mutation happened within epitope A and the site A that binding with histo-blood group antigens(HBGAs). The JN010 strain was GII.Pe/GII.4 genotype and genetically closest to the strains found in Osaka of Japan(GenBank accession number LC066046) and the strains in Zhongshan city of Guangdong (GenBank accession number KY407064) respectively according to ORF1 and ORF2 gene homologous and phylogenetic analysis.
Conclusions:The NoV GII.4 variant strain JN010 has occurred mutations in the key site of the epitope A and site A that bind with HBGAs, and maybe affect its antigenicity and interaction with HBGAs.
