Etiology analysis on a laboratory confirmed human rabies case with atypical symptoms
10.3760/cma.j.issn.1003-9279.2019.02.007
- VernacularTitle: 1例临床非典型狂犬病患儿的发现和病原特征分析
- Author:
Xingle LI
1
;
Chunhua ZHU
2
;
Dongxiao LI
1
;
Lingzhi LIU
3
;
Zhiquan HE
4
;
Xueyong HUANG
1
Author Information
1. Institute for Infectious Disease Control and Prevention, Henan Centers for Disease Control and Prevention, Zhengzhou 450016, China
2. Zhengzhou Jinshui District General Hospital, Zhengzhou 450014, China
3. Medicine Intensive Care Unit, Children′s Hospital Affiliated to Zhengzhou University, Zhengzhou 450018, China
4. College of Public Health, Zhengzhou University, Zhengzhou 450001, China
- Publication Type:Journal Article
- Keywords:
Rabies;
Rabies virus;
Reverse transcription-polymerase chain reaction (RT-PCR);
Phylogenetic analysis
- From:
Chinese Journal of Experimental and Clinical Virology
2019;33(2):148-151
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To investigate and analyze the clinical features, epidemiologic information and pathogenic characteristics of a rabies patient.
Methods:Clinical data of the patient(boy) was collected and epidemiological survey was conducted, fluorescence quantitative reverse transcription-polymerase chain reaction (FQRT-PCR) and reverse transcription-polymerase chain reaction (RT-PCR) were used to detect the samples of saliva, cerebrospinal fluid (CSF), skin tissue with hair follicle at the back of the neck for rabies laboratory diagnosis.
Results:Early symptoms of the boy were vomiting, diarrhea, fever and irritability, followed by coma and death. The boy had nasal trauma one month ago and the domestic dog died of illness during the same period. He did not accept the rabies post-exposure prophylaxis (PEP). The result of the saliva sample was positive by FQRT-PCR. The predicted segments of the glycoprotein(G), nucleoprotein (N) genes of rabies virus were amplified from the positive saliva sample of the patient by RT-PCR. Compared with rabies virus strains in Henan province, the nucleotide homology and amino acid homology in G gene segment were 96.5%-98.8% and 96.5%-99.2% respectively.
Conclusions:The case was diagnosed in laboratory as rabies case. The pathogenic rabies virus strain was endemic in Henan province. The nasal trauma, the dead domestic dog were probably related to the infection of the boy.
