Genetic analysis of norovirus strains detected from sporadic cases in Anhui province, 2016-2017
10.3760/cma.j.issn.1003-9279.2019.02.005
- VernacularTitle: 2016—2017年安徽省诺如病毒感染的散发病例基因型别分析
- Author:
Yuan YUAN
1
;
Yonglin SHI
;
Yong SUN
;
Wanfu HU
;
Weiwei LI
;
Yinglu GE
Author Information
1. Anhui Provincial Center for Disease Control and Prevention, Key Laboratory for Medical and Health of the 13th Five-year Plan in Anhui Province, Hefei 230601, China
- Publication Type:Journal Article
- Keywords:
Diarrhea;
Norovirus;
Genotype;
Phylogenetic tree
- From:
Chinese Journal of Experimental and Clinical Virology
2019;33(2):136-141
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To analyze the genotype diversity and phylogenetic characterization of norovirus(NoV) in patients with diarrhea from Anhui province.
Methods:NoV positive fecal specimens from sentinel hospitals were collected from January, 2016 to December, 2017. The samples were detected by Real-time fluorescent quantitative PCR. Positive samples were of randomly selected and amplified by RT-PCR and the products were sequenced. Phylogenetic tree was constructed by the Neighbor-Joining method based on partial VP1 gene regions of NoV to perform phylogenetic analysis.
Results:A total of 263 NoV positive samples were genotyped, of which 239 belonged to genogroup II, 24 belonged to genogroup I. Fifty-five positive samples were successfully sequenced. There were 6 NoV GII genotypes, which included GII.2, 3, 4/Sydney_2012, 13, 17 and 21, while NV GII.17 and GII.4 were the dominant genotypes from 2016 to 2017. The predominant genotype was GII.4/Sydney 2012 (47.27%, 26/55), followed by GII.17 (23.64%, 13/55) and GII.2 (14.55%, 8/55). Phylogenetic tree showed that 26 strains belonged to genotype GII.4/Sydney 2012, NoV. The nucleotide homology among the 26 VP1 genes was 97.8% to 100%. Analysis of the partial VP1 genes of 26 strains showed that it shared the highest homology of 98.9% with the strain of GII.4Sydney2012 (GenBank ID: KU720515). However, the prevailing genotype in the Anhui province has shifted on two separate occasions, the GII.17 strain was dominant in 2016, and the GII.4/Sydney 2012 strain was dominant in 2017.
Conclusions:NoV GII was the major pathogen causing sporadic diarrhea in Anhui province during from 2016 to 2017, the genotypes are widely distributed, and shifted into the two predominant strains.
