Molecular epidemiology of norovirus among infants with diarrhea in Chaoyang district, Beijing from 2011 to 2017

Yang Jiao; Furong HE; Yan Gao; Xiao QI; Shiyao Zhang; Lin GU; Yusong Zhang; Jianhong Zhao; Yue Zhang; Shen GE; Jialiang DU; Lingli Sun

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Molecular epidemiology of norovirus among infants with diarrhea in Chaoyang district, Beijing from 2011 to 2017

VernacularTitle: 北京市朝阳区2011—2017年5岁以下患儿腹泻标本中诺如病毒分子流行病学特征
Author: Yang JIAO ¹ ; Furong HE ¹ ; Yan GAO ¹ ; Xiao QI ¹ ; Shiyao ZHANG ¹ ; Lin GU ¹ ; Yusong ZHANG ¹ ; Jianhong ZHAO ¹ ; Yue ZHANG ¹ ; Shen GE ¹ ; Jialiang DU ² ; Lingli SUN ¹
Author Information

1. Microbiology Laboratory, Beijing Chaoyang District Centre for Disease Control and Prevention, Beijing 100021, China
2. Institute for Biological Products Control, National Institutes for Food and Drug Control, Beijing 102629, China
Publication Type:Journal Article
Keywords: Norovirus; Viral diarrhea; Genotype; Phylogenetic analysis
From: Chinese Journal of Experimental and Clinical Virology 2019;33(2):125-130
CountryChina
Language:Chinese
Abstract: Objective:To investigate the genetic characteristics of human norovirus (NoV) among infants under 5 years of age with diarrhea in Chaoyang District, Beijing from 2011 to 2017.
Methods:NoV-positive stool samples were collected from 2011 to 2017 in this region. The partial RdRp and VP1 genes were amplified and sequenced. Multi-sequence alignment was performed and phylogenetic tree was constructed using Mega software.
Results:A total of 151 samples were sequenced and analyzed. The ratio of male and female was 2.28∶1 with mean age of 1.72 years. Fourteen NoV subtypes were detected, including GII.Pe/GII.4 (47.68%), GII.P12/GII.3 (20.53%), GII.P4/GII.4 (17.22%), GII.P16/GII.2 (3.31%), GII.P12/GII.12 (1.99%), GII.P17/GII.17 (1.99%), GII.P16/GII.13 (1.32%), GII.P7/GII.7 (1.32%), GII.P7/GII.6 (1.32%), GII.P2/GII.2 (0.66%), GII.P21/GII.21 (0.66%), GII.Pg/GII.12 (0.66%), GI.Pa/GI.3 (0.66%) and GI.P6/GI.6 (0.66%).
Conclusions:NoV genetic diversity was found among infants under 5 with diarrhea in Chaoyang district, Beijing. The subtypes from surveillance and those from epidemics occurred in chronological order. The surveillance should be strengthened for early detection of new subtype for monitoring the epidemic and vaccine design.