Determination of Cortisol in Saliva by Liquid Chromatography Tandem Mass Spectrometry

Xiaodong Liu; He Qiao; Xiangjuan Meng; Chao Wang; Xiaowen Ding; Dongsheng Niu; Jue LI

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Determination of Cortisol in Saliva by Liquid Chromatography Tandem Mass Spectrometry

VernacularTitle: 唾液中皮质醇测定的液相色谱-质谱法
Author: Xiaodong LIU ¹ ; He QIAO ^{1
,

2} ; Xiangjuan MENG ¹ ; Chao WANG ¹ ; Xiaowen DING ¹ ; Dongsheng NIU ¹ ; Jue LI ¹
Author Information

1. The Beijing Prevention and Treatment of Hospital of Occupational Disease for Chemical Industry, Beijing 100093, China
2. Beijing University of Chemical Technology, Beiijng100029, China
Publication Type:Journal Article
Keywords: Cortisol; Saliva; Liquid chromatography tandem mass spectrometry; Occupational stress
From: Chinese Journal of Industrial Hygiene and Occupational Diseases 2019;37(2):143-146
CountryChina
Language:Chinese
Abstract: Objective:To establish a liquid chromatography tandem mass spectrometry method to determine the cortisol in saliva.
Methods:Take 0.5 ml saliva sample into a 2 ml centrifuge tube, add excess sodium chloride and 1ml acetonitrile to it, then vortex for 3 min, centrifuge for 10 min at 15 000 r/min, and take 800 μl of the upper layer to another centrifuge tube. Finally, the sample was concentrated by a vacuum concentrator and brought to 200 μl with the initial mobile phase. Then, the sample was analyzed by liquid chromatography tandem mass spectrometry. The target compound was quantified by external standard curve method.
Results:The linear range of the method was 0.02-5.00 ng/ml, r=0.999 9, the method limit of the detection was 0.002 ng/ml, the method limit of quantitative was 0.02 ng/ml, and the spiked recoveries were 89.60%-98.60%. The intra-assay precision was 1.90%-3.30%, and the inter-assay precision was 4.20%-9.00%; samples could be stored at -20 °C for at least 14 days. The determination of cortisol could not be interfered by other endogenous substances in the sample.
Conclusion:The method is simple in pretreatment, high sensitivity, good reproducibility and good recovery, and it is suitable for the quantitative analysis of cortisol in saliva for normal and occupationally stressed populations.