Therapeutic effect of dihydroartemisinin on pulmonary fibrosis in rats with dust
10.3760/cma.j.issn.1001-9391.2019.02.003
- VernacularTitle: 双氢青蒿素对染尘大鼠肺纤维化治疗作用的研究
- Author:
Yijiao ZHENG
1
,
2
;
Xia LI
3
;
Lin SUN
1
,
2
;
Jingwen GUO
1
,
2
Author Information
1. School of Medicine and Life Science, Universicy of Jinan-Shandong Academy of Medicine Sciences, Jinan 250062, China
2. Shandong Academy of Medical Sciences Shandong Academy of Occupational Health and Occupational Medicine, Jinan 250062, China
3. Shandong Academy of Medical Sciences Shandong Academy of Occupational Health and Occupational Medicine, Jinan 250062, China
- Publication Type:Journal Article
- Keywords:
Dihydroartemisinin;
Tetrandrine;
Rats;
Pulmonary fibrosis Found
- From:
Chinese Journal of Industrial Hygiene and Occupational Diseases
2019;37(2):96-103
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To observe the therapeutic effect of dihydroartemisinin on pulmonary fibrosis in rats exposed to dust, and compare the therapeutic effects of dihydroartemisinin and tetrandrine.
Methods:Sixty male Sprague-Dawley rats were randomly divided into control group, model group, treatment group 1 and treatment group 2, with 15 rats in each group. The model group and the treatment group were stained with disposable non-exposure silica tracheal instillation method. The drug was administered on the second day after the dust was applied. The treatment group was given with dihydroartemisinin 75 mg/kg, the treatment group was given tetrahexine 22 mg/kg, model group and control group were intragastrically administered with 1 ml of normal saline per 100 g of body mass. The drug was administered for 6 days per week for 28 days. Rats were sacrificed on the 7th, 14th and 28th day after dusting, and the lung tissues of rats were taken, detection of rat lung coefficient, ELISA was used to detect transforming growth factor-1(TGF-1)and Smad 2/3 in rat lung tissues, type I collagen (Col-I) expression level, pathological changes of rat lung tissue, immunohistochemical observation of rat lung tissue TGF-1 and Col-I protein expression. Statistial analysis was proformed with SPSS 19.0. The mean cornparis between graups wad perfomed using a completely randonized one-way (ANOVA).
Results:The expressions of TGF-1, Smad 2/3 and Col-I in the lung tissue of the treatment group were significantly lower than those in the model group (P< 0.05), but the difference between the treatment group 1 and the treatment group 2 Not statistically significant (P>0.05). The results of immunohistochemistry showed that the TGF-1 and Col-I proteins in the interstitial of the model group showed strong positive expression. The expression of TGF-1 and Col-I protein in the lung interstitial of the treated group was weaker than that of the model group.
Conclusion:Dihydroartemisinin can down-regulate the level of inflammatory cytokines in rat lung tissue. It is possible to inhibit pulmonary fibrosis by intervening in the TGF-β1/Smad 2/3 signaling pathway, but the therapeutic effect between DHA and tetrandrine is not significantly different in a short time.
