Pathological study on mast cells and their released protease and transforming growth factor-β1 in lymphedema skin tissue
10.3760/cma.j.issn.1009-4598.2019.01.016
- VernacularTitle: 淋巴水肿皮肤组织中肥大细胞及其释放的蛋白酶和转化生长因子-β1之间的相关病理研究
- Author:
Di SUN
1
;
Ziyou YU
;
Jiajia CHEN
;
Li WANG
;
Linghua HAN
;
Ningfei LIU
Author Information
1. Department of Plastic and Reconstructive Surgery, Shanghai Ninth People′s Hospital, Shanghai JiaotongUniversity School of Medicine, Shanghai 20001, China
- Publication Type:Journal Article
- Keywords:
Lymphedema;
Mast cell;
Transforming growth factor-β
1
- From:
Chinese Journal of Plastic Surgery
2019;35(1):68-75
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To explore the relationship between protease-chymase secreted by mast cells, and activated transforming growth factor-β1(TGF-β1), in skin with secondary lymphedema(SLE)in lower extremity, so as toidentify the key factors in fibrosis of lymphedema.
Methods:In this study, the affected limb skin of 7 SLE patients was includedas the experimental group, and normal skin tissue of the lower limb of 7 volunteers was used as controls. The skin samples were assayed by Masson staining, and the expressions of chymase and TGF-β1 were assayed by immunohistochemistry, immunofluorescent staining and enzyme-linked immunosorbent assay.
Results:There was obvious fibrosis in the skin of lower extremity in patients with lymphedema. The number of MCs andthe expressions of chymase, latency-associated peptide TGF-β1 (LAP TGF-β1) and TGF-β1 were all significantly increased in fibrotic skin in lymphedema, compared with those in normal skin. At the same time, the chymase-containing mast cells accumulated in the lymphatic vessels, with higher expression of TGF-β1.
Conclusions:The expression of chymase and TGF-β1 was significantly increased in the fibrotic skin insecondary lower extremity lymphedema. The increased expression of chymase in the skin may activate more TGF-β1 expression, and the increased TGF-β1 may promote skin fibrosis in SLE.
